BVES sirna

BVES siRNA (Human)

Cat. Num. AAA8232948

• Gene Names: BVES; POP1; HBVES; POPDC1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BVES; BVES siRNA (Human); POP1; POPDC1; Blood vessel epicardial substance; hBVES; Popeye domain-containing protein 1; Popeye protein 1; BVES sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: BVES siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 360

• Applicable Applications for BVES sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BVES gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BVES sirna

siRNA to inhibit BVES expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 313760596
• NCBI GeneID: 11149
• NCBI Accession #: NP_001186492.1
• NCBI GenBank Nucleotide #: NM_001199563.1
• UniProt Accession #: Q8NE79; Q5T550; Q5T551; Q8IWC6; Q9HBV0; Q9UNG6; A8K1R4; E1P5D8
• Molecular Weight: 41,451 Da
• NCBI Official Full Name: blood vessel epicardial substance
• NCBI Official Synonym Full Names: blood vessel epicardial substance
• NCBI Official Symbol: BVES
• NCBI Official Synonym Symbols: POP1; HBVES; POPDC1
• NCBI Protein Information: blood vessel epicardial substance
• UniProt Protein Name: Blood vessel epicardial substance
• Protein Family: Blood vessel epicardial substance
• UniProt Gene Name: BVES
• UniProt Synonym Gene Names: POP1; POPDC1; hBVES; Popeye protein 1
• UniProt Entry Name: POPD1_HUMAN

NCBI Description

This gene encodes a member of the POP family of proteins containing three putative transmembrane domains. This gene is expressed in cardiac and skeletal muscle and may play an important role in development of these tissues. The mouse ortholog may be involved in the regeneration of adult skeletal muscle and may act as a cell adhesion molecule in coronary vasculogenesis. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

BVES: Cell adhesion molecule involved in the establishment and/or maintenance of cell integrity. Involved in the formation and regulation of the tight junction (TJ) paracellular permeability barrier in epithelial cells. Plays a role in VAMP3- mediated vesicular transport and recycling of different receptor molecules through its interaction with VAMP3. Plays a role in the regulation of cell shape and movement by modulating the Rho-family GTPase activity through its interaction with ARHGEF25/GEFT. Induces primordial adhesive contact and aggregation of epithelial cells in a Ca(2+)-independent manner. Also involved in striated muscle regeneration and repair and in the regulation of cell spreading. Belongs to the popeye family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: tight junction; plasma membrane; integral to membrane; lateral plasma membrane

Molecular Function: cAMP binding; structural molecule activity

Biological Process: vesicle-mediated transport; regulation of cell shape; regulation of membrane potential; positive regulation of locomotion; muscle development; regulation of heart rate; hemopoietic progenitor cell differentiation; positive regulation of receptor recycling