Human IL-4 ELISA Kit | IL4 elisa kit

Human IL-4 Quick ELISA Kit

Cat. Num. AAA1753910

• Gene Names: IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1
• Reactivity: Human
• Synonyms: IL-4; Human IL-4 Quick ELISA Kit; IL-4|B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra; IL4 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human IL4

• Assay Type: Colorimetric sandwich
• Samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 15.6pg/ml-1,000pg/ml
• Sensitivity: <1.5pg/ml
• Capture/Detection Antibodies: Capture antibody- monoclonal antibody from mouse|Detection antibody- polyclonal antibody from goat
• Sample Data: Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000; O.D.|; 0.040|0.148|0.238|0.416|0.755|1.314|2.094|2.570
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|42|224|543|; Standard deviation|2.26|12.09|25.52|; CV(%)|5.4%|5.4%|4.7%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|33|199|537; Standard deviation|2.14|10.94|29.53; CV(%)|6.5%|6.6%|5.5%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 42|35|40|33|37|1.59|4.3%; Sample 2: 224|195|213|207|209|8.77|4.2%; Sample 3: 543|445|515|487|497|27.33|5.5%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: E Coli; Immunogen sequence: H25-S153
• Anticoagulant: heparin or EDTA
• Benefits: The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human IL-4 with <1. 5pg/ml sensitivity. Format: 96-well plate with removable strips.; This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
• Principle: Human IL4 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL4 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL4. Te detection antibody is a HRP linked goat polyclonal antibody specific for IL4. The kit contains recombinant Human IL4 with immunogen: Expression system for standard: E Coli; Immunogen sequence: H25-S153. The kit is analytically validated with ready to use reagents.; ; To measure Human IL4, add standards and samples to the wells, then add HRP linked detection antibody. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human IL4 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IL4 in the sample.
• Assay Procedure Step 1: 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml
• TMB Incubation Time: 15
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Testing Data

Related Product Information for IL4 elisa kit

Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.

Product Categories/Family for IL4 elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 4504669
• NCBI GeneID: 3565
• NCBI Accession #: NP_000580.1
• NCBI GenBank Nucleotide #: NM_000589.3
• UniProt Accession #: P05112; Q14630
• Molecular Weight: 17,492 Da
• NCBI Official Full Name: interleukin-4 isoform 1
• NCBI Official Synonym Full Names: interleukin 4
• NCBI Official Symbol: IL4
• NCBI Official Synonym Symbols: BSF1; IL-4; BCGF1; BSF-1; BCGF-1
• NCBI Protein Information: interleukin-4; binetrakin; pitrakinra; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1
• UniProt Protein Name: Interleukin-4
• Protein Family: Interleukin
• UniProt Gene Name: IL4
• UniProt Synonym Gene Names: IL-4; BSF-1
• UniProt Entry Name: IL4_HUMAN

NCBI Description

The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Cell cycle regulation; Motility/polarity/chemotaxis; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; external side of plasma membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; interleukin-4 receptor binding

Biological Process: regulation of isotype switching; negative regulation of osteoclast differentiation; positive regulation of isotype switching to IgG isotypes; positive regulation of transcription, DNA-dependent; regulation of proton transport; microglial cell activation; female pregnancy; positive regulation of activated T cell proliferation; chemotaxis; response to organic cyclic substance; positive regulation of interleukin-10 production; positive regulation of isotype switching to IgE isotypes; B cell costimulation; positive regulation of MHC class II biosynthetic process; connective tissue growth factor biosynthetic process; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of interleukin-13 production; negative regulation of macrophage activation; response to nutrient; response to drug; cholesterol metabolic process; negative regulation of chronic inflammatory response; regulation of immune response; T-helper 2 type immune response; B cell activation; regulation of phosphorylation; negative regulation of nitric oxide biosynthetic process; negative regulation of acute inflammatory response; defense response to protozoan; T-helper 1 cell lineage commitment; positive regulation of chemokine biosynthetic process; response to ethanol; innate immune response in mucosa; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of T cell differentiation; retina development in camera-type eye; response to cytokine stimulus; B cell differentiation; cellular defense response; positive regulation of transcription factor activity; immune response; positive regulation of transcription from RNA polymerase II promoter; T-helper 2 cell differentiation; negative regulation of transcription, DNA-dependent; positive regulation of defense response to virus by host; negative regulation of apoptosis

Disease: Stroke, Ischemic