ATP6V0A4 sirna

ATP6V0A4 siRNA (Human)

Cat. Num. AAA8214915

• Gene Names: ATP6V0A4; A4; STV1; VPH1; VPP2; RTA1C; RTADR; ATP6N2; RDRTA2; ATP6N1B
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATP6V0A4; ATP6V0A4 siRNA (Human); ATP6N1B; ATP6N2; V-type proton ATPase 116 kDa subunit a isoform 4; V-ATPase 116 kDa isoform a4; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4; Vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform; ATP6V0A4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ATP6V0A4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 840

• Applicable Applications for ATP6V0A4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ATP6V0A4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATP6V0A4 sirna

siRNA to inhibit ATP6V0A4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 85386053
• NCBI GeneID: 50617
• NCBI Accession #: NP_065683.2
• NCBI GenBank Nucleotide #: NM_020632.2
• UniProt Accession #: Q9HBG4; Q32M47; A4D1R4; A8KA80
• Molecular Weight: 96,386 Da
• NCBI Official Full Name: V-type proton ATPase 116 kDa subunit a isoform 4
• NCBI Official Synonym Full Names: ATPase, H+ transporting, lysosomal V0 subunit a4
• NCBI Official Symbol: ATP6V0A4
• NCBI Official Synonym Symbols: A4; STV1; VPH1; VPP2; RTA1C; RTADR; ATP6N2; RDRTA2; ATP6N1B
• NCBI Protein Information: V-type proton ATPase 116 kDa subunit a isoform 4
• UniProt Protein Name: V-type proton ATPase 116 kDa subunit a isoform 4
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V0A4
• UniProt Synonym Gene Names: ATP6N1B; ATP6N2; V-ATPase 116 kDa isoform a4
• UniProt Entry Name: VPP4_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6V0A4: Part of the proton channel of the V-ATPase that is involved in normal vectorial acid transport into the urine by the kidney. Defects in ATP6V0A4 are the cause of distal renal tubular acidosis with preserved hearing (RTADR). RTADR is an autosomal recessive form of distal renal tubular acidosis (dRTA), a group of disorders characterized by functional failure of alpha- intercalated cells of the cortical collecting duct of the distal nephron, where vectorial proton transport is required for urinary acidification. Functional failure of alpha-intercalated cells results in metabolic acidosis accompanied by disturbances of potassium balance, urinary calcium solubility, bone physiology and growth. Belongs to the V-ATPase 116 kDa subunit family.

Protein type: Membrane protein, integral; Energy Metabolism - oxidative phosphorylation; Transporter, ion channel; Transporter; Membrane protein, multi-pass; Transporter, iron

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: phagocytic vesicle membrane; brush border membrane; apical part of cell; lysosomal membrane; apical plasma membrane; integral to membrane; plasma membrane; endosome membrane; vacuolar proton-transporting V-type ATPase complex; endosome

Molecular Function: protein binding; hydrogen ion transporting ATPase activity, rotational mechanism; ATPase binding

Biological Process: interaction with host; vacuolar acidification; proton transport; ossification; ATP synthesis coupled proton transport; sensory perception of sound; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; regulation of pH; transferrin transport; excretion; transmembrane transport

Disease: Renal Tubular Acidosis, Distal, Autosomal Recessive