Rabbit anti-Human ATP6V0A4 Polyclonal Antibody | anti-ATP6V0A4 antibody

ATP6V0A4 Antibody

Cat. Num. AAA7046417

• Gene Names: ATP6V0A4; A4; STV1; VPH1; VPP2; RTA1C; RTADR; ATP6N2; RDRTA2; ATP6N1B
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: ATP6V0A4; Polyclonal Antibody; ATP6V0A4 Antibody; V-type proton ATPase 116 kDa subunit a isoform 4; V-ATPase 116 kDa isoform a4; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4; Vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform; ATP6N1B; ATP6N2; anti-ATP6V0A4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 840

• Applicable Applications for anti-ATP6V0A4 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: IHC: 1:20-1:200
• Species: Human
• Immunogen: Recombinant human V-type proton ATPase 116 kDa subunit a isoform 4 protein (N-280AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-69096 / sc-69099
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7046417 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS7046417 at dilution 1:100)

Related Product Information for anti-ATP6V0A4 antibody

Part of the proton channel of the V-ATPase that is involved in normal vectorial acid transport into the urine by the kidney.

NCBI and Uniprot Product Information

• NCBI GI #: 85386053
• NCBI GeneID: 50617
• NCBI Accession #: NP_065683.2
• NCBI GenBank Nucleotide #: NM_020632.2
• UniProt Accession #: Q9HBG4; Q32M47; A4D1R4; A8KA80
• Molecular Weight: 96,386 Da
• NCBI Official Full Name: V-type proton ATPase 116 kDa subunit a isoform 4
• NCBI Official Synonym Full Names: ATPase H+ transporting V0 subunit a4
• NCBI Official Symbol: ATP6V0A4
• NCBI Official Synonym Symbols: A4; STV1; VPH1; VPP2; RTA1C; RTADR; ATP6N2; RDRTA2; ATP6N1B
• NCBI Protein Information: V-type proton ATPase 116 kDa subunit a isoform 4
• UniProt Protein Name: V-type proton ATPase 116 kDa subunit a isoform 4
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V0A4
• UniProt Synonym Gene Names: ATP6N1B; ATP6N2; V-ATPase 116 kDa isoform a4
• UniProt Entry Name: VPP4_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6V0A4: Part of the proton channel of the V-ATPase that is involved in normal vectorial acid transport into the urine by the kidney. Defects in ATP6V0A4 are the cause of distal renal tubular acidosis with preserved hearing (RTADR). RTADR is an autosomal recessive form of distal renal tubular acidosis (dRTA), a group of disorders characterized by functional failure of alpha- intercalated cells of the cortical collecting duct of the distal nephron, where vectorial proton transport is required for urinary acidification. Functional failure of alpha-intercalated cells results in metabolic acidosis accompanied by disturbances of potassium balance, urinary calcium solubility, bone physiology and growth. Belongs to the V-ATPase 116 kDa subunit family.

Protein type: Transporter; Membrane protein, integral; Energy Metabolism - oxidative phosphorylation; Transporter, iron; Membrane protein, multi-pass; Transporter, ion channel

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: apical part of cell; apical plasma membrane; brush border membrane; endosome; endosome membrane; lysosomal membrane; phagocytic vesicle membrane; plasma membrane; vacuolar proton-transporting V-type ATPase complex

Molecular Function: ATPase binding; hydrogen ion transporting ATPase activity, rotational mechanism; protein binding

Biological Process: ATP synthesis coupled proton transport; excretion; insulin receptor signaling pathway; ossification; proton transport; regulation of pH; sensory perception of sound; transferrin transport; vacuolar acidification

Disease: Renal Tubular Acidosis, Distal, Autosomal Recessive