APOBEC3H sirna

APOBEC3H siRNA (Human)

Cat. Num. AAA8223330

• Gene Names: APOBEC3H; A3H; ARP10; ARP-10
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APOBEC3H; APOBEC3H siRNA (Human); DNA dC->dU-editing enzyme APOBEC-3H; APOBEC-related protein 10; ARP-10; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3H; A3H; APOBEC3H sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: APOBEC3H siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 182

• Applicable Applications for APOBEC3H sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human APOBEC3H gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APOBEC3H sirna

siRNA to inhibit APOBEC3H expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 669250885
• NCBI GeneID: 164668
• NCBI Accession #: NP_001159474.2
• NCBI GenBank Nucleotide #: NM_001166002.2
• UniProt Accession #: Q6NTF7; Q5JYL9; Q6IC87; B0QYP0; B0QYP1; B7TQM5; E9PF38
• Molecular Weight: 17,782 Da
• NCBI Official Full Name: DNA dC->dU-editing enzyme APOBEC-3H isoform SV-182
• NCBI Official Synonym Full Names: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3H
• NCBI Official Symbol: APOBEC3H
• NCBI Official Synonym Symbols: A3H; ARP10; ARP-10
• NCBI Protein Information: DNA dC->dU-editing enzyme APOBEC-3H
• UniProt Protein Name: DNA dC->dU-editing enzyme APOBEC-3H
• Protein Family: DNA dC->dU-editing enzyme
• UniProt Gene Name: APOBEC3H
• UniProt Synonym Gene Names: ARP-10; A3H
• UniProt Entry Name: ABC3H_HUMAN

NCBI Description

This gene encodes a member of the apolipoprotein B mRNA-editing enzyme catalytic polypeptide 3 family of proteins. The encoded protein is a cytidine deaminase that has antiretroviral activity by generating lethal hypermutations in viral genomes. Polymorphisms and alternative splicing in this gene influence its antiretroviral activity and are associated with increased resistence to human immunodeficiency virus type 1 infection in certain populations. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009]

Uniprot Description

APOBEC3H: DNA cytidine deaminase which may provide cellular innate resistance to a specific panel of genetic invaders including endogenous retroelements and a subset of viruses. Belongs to the cytidine and deoxycytidylate deaminase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.5.4.-

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; cytidine deaminase activity

Biological Process: negative regulation of retroviral genome replication; innate immune response; cytidine deamination; negative regulation of viral reproduction; defense response to virus