APH1B sirna

APH1B siRNA (Human)

Cat. Num. AAA8218321

• Gene Names: APH1B; PSFL; APH-1B; PRO1328; TAAV688
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APH1B; APH1B siRNA (Human); PSFL; Gamma-secretase subunit APH-1B; APH-1b; Aph-1beta; Presenilin-stabilization factor-like; APH1B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: APH1B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 216

• Applicable Applications for APH1B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human APH1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APH1B sirna

siRNA to inhibit APH1B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 224548987
• NCBI GeneID: 83464
• NCBI Accession #: NP_001139118.1
• NCBI GenBank Nucleotide #: NM_001145646.1
• UniProt Accession #: Q8WW43; Q564N3; Q6UWQ1; Q9H0S0; A8K589
• Molecular Weight: 24,282 Da
• NCBI Official Full Name: gamma-secretase subunit APH-1B isoform 2
• NCBI Official Synonym Full Names: APH1B gamma secretase subunit
• NCBI Official Symbol: APH1B
• NCBI Official Synonym Symbols: PSFL; APH-1B; PRO1328; TAAV688
• NCBI Protein Information: gamma-secretase subunit APH-1B
• UniProt Protein Name: Gamma-secretase subunit APH-1B
• Protein Family: Gamma-secretase
• UniProt Gene Name: APH1B
• UniProt Synonym Gene Names: PSFL; APH-1b
• UniProt Entry Name: APH1B_HUMAN

NCBI Description

This gene encodes a multi-pass transmembrane protein that is a functional component of the gamma-secretase complex, which also contains presenilin and nicastrin. This protein represents a stabilizing cofactor for the presenilin holoprotein in the complex. The gamma-secretase complex catalyzes the cleavage of integral proteins such as notch receptors and beta-amyloid precursor protein. [provided by RefSeq, Sep 2011]

Uniprot Description

APH1B: Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. Probably present in a minority of gamma-secretase complexes compared to APH1A. Belongs to the APH-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: transport vesicle; integral to membrane; plasma membrane

Molecular Function: peptidase activity; protein binding

Biological Process: positive regulation of catalytic activity; axon guidance; Notch signaling pathway; membrane protein intracellular domain proteolysis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; ephrin receptor signaling pathway; Notch receptor processing; protein processing