APH1A sirna

APH1A siRNA (Human)

Cat. Num. AAA8232410

• Gene Names: APH1A; APH-1; APH-1A; CGI-78; 6530402N02Rik
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APH1A; APH1A siRNA (Human); PSF; Gamma-secretase subunit APH-1A; APH-1a; Aph-1alpha; Presenilin-stabilization factor; APH1A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: APH1A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 265

• Applicable Applications for APH1A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human APH1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APH1A sirna

siRNA to inhibit APH1A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 117606362
• NCBI GeneID: 51107
• NCBI Accession #: NP_001071096.1
• NCBI GenBank Nucleotide #: NM_001077628.2
• UniProt Accession #: Q96BI3; Q5TB22; Q5TB23; Q969R6; Q9BVG0; Q9Y386; B4DQK0
• Molecular Weight: 20,977 Da
• NCBI Official Full Name: gamma-secretase subunit APH-1A isoform 1
• NCBI Official Synonym Full Names: APH1A gamma secretase subunit
• NCBI Official Symbol: APH1A
• NCBI Official Synonym Symbols: APH-1; APH-1A; CGI-78; 6530402N02Rik
• NCBI Protein Information: gamma-secretase subunit APH-1A
• UniProt Protein Name: Gamma-secretase subunit APH-1A
• Protein Family: Gamma-secretase
• UniProt Gene Name: APH1A
• UniProt Synonym Gene Names: PSF; APH-1a
• UniProt Entry Name: APH1A_HUMAN

NCBI Description

This gene encodes a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product, or the paralogous anterior pharynx defective 1 homolog B (APH1B), along with the presenilin, nicastrin, and presenilin enhancer-2 proteins. The precise function of this seven-transmembrane-domain protein is unknown though it is suspected of facilitating the association of nicastrin and presenilin in the gamma secretase complex as well as interacting with substrates of the gamma secretase complex prior to their proteolytic processing. Polymorphisms in a promoter region of this gene have been associated with an increased risk for developing sporadic Alzheimer's disease. Alternative splicing results in multiple protein-coding and non-protein-coding transcript variants. [provided by RefSeq, Aug 2011]

Uniprot Description

APH1A: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. Belongs to the APH-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Endoplasmic reticulum; Apoptosis; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p36.13-q31.3

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; membrane; integral to plasma membrane; endoplasmic reticulum; plasma membrane

Molecular Function: protein binding; endopeptidase activity

Biological Process: positive regulation of catalytic activity; axon guidance; amyloid precursor protein catabolic process; Notch signaling pathway; membrane protein intracellular domain proteolysis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; membrane protein ectodomain proteolysis; ephrin receptor signaling pathway; Notch receptor processing; protein processing; metanephros development