Mouse Gamma-secretase subunit APH-1A ELISA Kit | Aph1a elisa kit

Mouse Gamma-secretase subunit APH-1A ELISA Kit

Cat. Num. AAA762114

• Gene Names: APH1A; APH-1; APH-1A; CGI-78; 6530402N02Rik
• Reactivity: Mouse
• Synonyms: Gamma-secretase subunit APH-1A; Mouse Gamma-secretase subunit APH-1A ELISA Kit; Aph1a/6530402N02Rik/anterior pharynx defective 1 homolog A (C. elegans)/APH-1/APH-1A/APH-1a/aph-1alpha/Aph-1alpha/CGI-78/Presenilin-stabilization factor/PSF; Aph1a elisa kit

• Reactivity: Mouse
• Sequence Length: 195

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.56-100ng/ml
• Sensitivity: 0.938ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Aph1a elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Aph1a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Aph1a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Aph1a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Aph1a can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 344313186
• NCBI GeneID: 51107
• NCBI Accession #: NP_001230701.1
• NCBI GenBank Nucleotide #: NM_001243772.1
• Molecular Weight: 20,977 Da
• NCBI Official Full Name: gamma-secretase subunit APH-1A isoform 4
• NCBI Official Synonym Full Names: aph-1 homolog A, gamma secretase subunit
• NCBI Official Symbol: APH1A
• NCBI Official Synonym Symbols: APH-1; APH-1A; CGI-78; 6530402N02Rik
• NCBI Protein Information: gamma-secretase subunit APH-1A
• UniProt Protein Name: Gamma-secretase subunit APH-1A
• Protein Family: Gamma-secretase
• UniProt Gene Name: APH1A
• UniProt Synonym Gene Names: PSF; APH-1a
• UniProt Entry Name: APH1A_HUMAN

NCBI Description

This gene encodes a component of the gamma secretase complex that cleaves integral membrane proteins such as Notch receptors and beta-amyloid precursor protein. The gamma secretase complex contains this gene product, or the paralogous anterior pharynx defective 1 homolog B (APH1B), along with the presenilin, nicastrin, and presenilin enhancer-2 proteins. The precise function of this seven-transmembrane-domain protein is unknown though it is suspected of facilitating the association of nicastrin and presenilin in the gamma secretase complex as well as interacting with substrates of the gamma secretase complex prior to their proteolytic processing. Polymorphisms in a promoter region of this gene have been associated with an increased risk for developing sporadic Alzheimer's disease. Alternative splicing results in multiple protein-coding and non-protein-coding transcript variants. [provided by RefSeq, Aug 2011]

Uniprot Description

APH1A: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. Belongs to the APH-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Apoptosis; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 1p36.13-q31.3

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; Golgi apparatus; integral to plasma membrane; membrane; plasma membrane

Molecular Function: endopeptidase activity; protein binding

Biological Process: amyloid precursor protein catabolic process; axon guidance; ephrin receptor signaling pathway; membrane protein ectodomain proteolysis; membrane protein intracellular domain proteolysis; metanephros development; nerve growth factor receptor signaling pathway; Notch receptor processing; Notch signaling pathway; positive regulation of apoptosis; positive regulation of catalytic activity; protein processing