AMFR sirna

AMFR siRNA (Human)

Cat. Num. AAA8224941

• Gene Names: AMFR; GP78; RNF45
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AMFR; AMFR siRNA (Human); RNF45; E3 ubiquitin-protein ligase AMFR; Autocrine motility factor receptor; AMF receptor; RING finger protein 45; gp78; AMFR sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AMFR siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 643

• Applicable Applications for AMFR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AMFR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AMFR sirna

siRNA to inhibit AMFR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21071001
• NCBI GeneID: 267
• NCBI Accession #: NP_001135.3
• NCBI GenBank Nucleotide #: NM_001144.5
• UniProt Accession #: Q9UKV5; P26442; Q8IZ70
• Molecular Weight: 72,996 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase AMFR
• NCBI Official Synonym Full Names: autocrine motility factor receptor, E3 ubiquitin protein ligase
• NCBI Official Symbol: AMFR
• NCBI Official Synonym Symbols: GP78; RNF45
• NCBI Protein Information: E3 ubiquitin-protein ligase AMFR
• UniProt Protein Name: E3 ubiquitin-protein ligase AMFR
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: AMFR
• UniProt Synonym Gene Names: RNF45; AMF receptor
• UniProt Entry Name: AMFR_HUMAN

NCBI Description

This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins. [provided by RefSeq, Feb 2012]

Uniprot Description

AMFR: E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97- AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97- AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis. Interacts with RNF5. Also forms an ERAD complex containing VCP/p97, NGLY1; PSMC1; SAKS1 AND RAD23B required for coupling retrotranslocation, ubiquitination and deglycosylation. Interacts with DRL1. Interacts (through a region distinct from the RING finger) with UBE2G2/UBC7. Component of the VCP/p97-AMFR/gp78 complex that enhances VCP/p97 binding to polyubiquitinated proteins for their degradation by the endoplasmic reticulum-associated degradation (ERAD) pathway. Interacts (via the VIM) with VCP/p97. Interacts (via its membrane domain) with INSIG1; the interaction initiates the sterol-mediated ubiquitination and degradation of HMGCR by the ERAD pathway. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Ubiquitin conjugating system; Endoplasmic reticulum; EC 6.3.2.19; EC 6.3.2.-; Membrane protein, integral; Ligase; Membrane protein, multi-pass; Receptor, misc.; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: endoplasmic reticulum membrane; growth cone; protein complex; cell soma; membrane; perinuclear region of cytoplasm; dendrite; integral to membrane; integral to endoplasmic reticulum membrane; nucleus

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; receptor activity; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; ER-associated protein catabolic process; learning and/or memory; protein polyubiquitination; unfolded protein response; cell motility; signal transduction; protein oligomerization; aging