ADRBK2 sirna

ADRBK2 siRNA (Human)

Cat. Num. AAA829542

• Gene Names: ADRBK2; GRK3; BARK2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADRBK2; ADRBK2 siRNA (Human); BARK2; GRK3; Beta-adrenergic receptor kinase 2; Beta-ARK-2; G-protein-coupled receptor kinase 3; ADRBK2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ADRBK2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 688

• Applicable Applications for ADRBK2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADRBK2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADRBK2 sirna

siRNA to inhibit ADRBK2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 148539879
• NCBI GeneID: 157
• NCBI Accession #: NP_005151.2
• NCBI GenBank Nucleotide #: NM_005160.3
• UniProt Accession #: P35626; Q9UGW9
• Molecular Weight: 79,710 Da
• NCBI Official Full Name: beta-adrenergic receptor kinase 2
• NCBI Official Synonym Full Names: adrenergic, beta, receptor kinase 2
• NCBI Official Symbol: ADRBK2
• NCBI Official Synonym Symbols: GRK3; BARK2
• NCBI Protein Information: beta-adrenergic receptor kinase 2
• UniProt Protein Name: Beta-adrenergic receptor kinase 2
• Protein Family: Beta-adrenergic receptor kinase
• UniProt Gene Name: ADRBK2
• UniProt Synonym Gene Names: BARK2; GRK3; Beta-ARK-2
• UniProt Entry Name: ARBK2_HUMAN

NCBI Description

The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008]

Uniprot Description

GRK3: Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Protein type: Kinase, protein; EC 2.7.11.15; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; GRK family; BARK subfamily

Chromosomal Location of Human Ortholog: 22q12.1

Molecular Function: G-protein coupled receptor kinase activity; beta-adrenergic receptor kinase activity; ATP binding; protein kinase activity

Biological Process: receptor internalization; signal transduction; protein amino acid phosphorylation