ADH1C sirna

ADH1C siRNA (Human)

Cat. Num. AAA8218824

• Gene Names: ADH1C; ADH3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADH1C; ADH1C siRNA (Human); ADH3; Alcohol dehydrogenase 1C; Alcohol dehydrogenase subunit gamma; ADH1C sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ADH1C siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 375

• Applicable Applications for ADH1C sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADH1C gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADH1C sirna

siRNA to inhibit ADH1C expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4501933
• NCBI GeneID: 126
• NCBI Accession #: NP_000660.1
• NCBI GenBank Nucleotide #: NM_000669.4
• UniProt Accession #: P00326; Q4PJ18; Q5WRV0; Q6LBW4; Q6NWV0; Q6NZA7
• Molecular Weight: 39,868 Da
• NCBI Official Full Name: alcohol dehydrogenase 1C
• NCBI Official Synonym Full Names: alcohol dehydrogenase 1C (class I), gamma polypeptide
• NCBI Official Symbol: ADH1C
• NCBI Official Synonym Symbols: ADH3
• NCBI Protein Information: alcohol dehydrogenase 1C
• UniProt Protein Name: Alcohol dehydrogenase 1C
• Protein Family: Alcohol dehydrogenase
• UniProt Gene Name: ADH1C
• UniProt Synonym Gene Names: ADH3
• UniProt Entry Name: ADH1G_HUMAN

NCBI Description

This gene encodes class I alcohol dehydrogenase, gamma subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq, Jul 2008]

Uniprot Description

ADH1C: class I alcohol dehydrogenase, gamma subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq, Jul 2008]

Protein type: EC 1.1.1.1; Mitochondrial; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - metabolism by cytochrome P450; Amino Acid Metabolism - tyrosine; Lipid Metabolism - fatty acid

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytosol

Molecular Function: zinc ion binding; alcohol dehydrogenase activity

Biological Process: xenobiotic metabolic process; ethanol oxidation

Disease: Alcohol Dependence; Parkinson Disease, Late-onset