Typical Testing Data/Standard Curve (for reference only)
Rat Alcohol dehydrogenase 1C (ADH1C) ELISA Kit | ADH1C elisa kit
Rat Alcohol dehydrogenase 1C (ADH1C) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ADH1C ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ADH1C antibody and an ADH1C-HRP conjugate. The assay sample and buffer are incubated together with ADH1C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADH1C concentration since ADH1C from samples and ADH1C-HRP conjugate compete for the anti-ADH1C antibody binding site. Since the number of sites is limited, as more sites are occupied by ADH1C from the sample, fewer sites are left to bind ADH1C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADH1C concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes class I alcohol dehydrogenase, gamma subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq, Jul 2008]
Uniprot Description
ADH1C: class I alcohol dehydrogenase, gamma subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq, Jul 2008]
Protein type: Oxidoreductase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Lipid Metabolism - fatty acid; Cofactor and Vitamin Metabolism - retinol; Amino Acid Metabolism - tyrosine; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.1.1.1; Mitochondrial
Chromosomal Location of Human Ortholog: 4q23
Cellular Component: cytosol
Molecular Function: zinc ion binding; alcohol dehydrogenase activity
Biological Process: xenobiotic metabolic process; ethanol oxidation
Disease: Alcohol Dependence; Parkinson Disease, Late-onset
Research Articles on ADH1C
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Product Notes
The Rat ADH1C adh1c (Catalog #AAA7228332) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7228332 ELISA Kit recognizes Rat ADH1C. It is sometimes possible for the material contained within the vial of "Alcohol dehydrogenase 1C (ADH1C), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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