ADCY10 sirna

ADCY10 siRNA (Human)

Cat. Num. AAA829729

• Gene Names: ADCY10; SAC; HCA2; SACI; Sacy; hsAC
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADCY10; ADCY10 siRNA (Human); SAC; Adenylate cyclase type 10; AH-related protein; Adenylate cyclase homolog; Germ cell soluble adenylyl cyclase; hsAC; sAC; Testicular soluble adenylyl cyclase; ADCY10 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ADCY10 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1457

• Applicable Applications for ADCY10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADCY10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADCY10 sirna

siRNA to inhibit ADCY10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 268370055
• NCBI GeneID: 55811
• NCBI Accession #: NP_001161221.1
• NCBI GenBank Nucleotide #: NM_001167749.2
• UniProt Accession #: Q96PN6; O95558; Q5R329; Q5R330; Q8WXV4; Q9NNX0; B4DZF0; F5GWS5
• Molecular Weight: 169,750 Da
• NCBI Official Full Name: adenylate cyclase type 10 isoform 2
• NCBI Official Synonym Full Names: adenylate cyclase 10 (soluble)
• NCBI Official Symbol: ADCY10
• NCBI Official Synonym Symbols: SAC; HCA2; SACI; Sacy; hsAC
• NCBI Protein Information: adenylate cyclase type 10
• UniProt Protein Name: Adenylate cyclase type 10
• Protein Family: Adenylate cyclase
• UniProt Gene Name: ADCY10
• UniProt Synonym Gene Names: SAC; hsAC; sAC
• UniProt Entry Name: ADCYA_HUMAN

NCBI Description

The protein encoded by this gene belongs to a distinct class of adenylyl cyclases that is soluble and insensitive to G protein or forskolin regulation. Activity of this protein is regulated by bicarbonate. Variation at this gene has been observed in patients with absorptive hypercalciuria. Alternatively spliced transcript variants encoding different isoforms have been observed. There is a pseudogene of this gene on chromosome 6. [provided by RefSeq, Jul 2014]

Uniprot Description

SAC: Soluble adenylyl cyclase that has a critical role in mammalian spermatogenesis. Produces the cAMP which mediates in part the cAMP-responsive nuclear factors indispensable for maturation of sperm in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. May be the bicarbonate sensor. Involved in ciliary beat regulation. Genetic variations in ADCY10 are a cause of susceptibility to hypercalciuria absorptive type 2 (HCA2). Absorptive hypercalciuria is a common type of hypercalciuria, a condition characterized by excessive urinary calcium excretion. Absorptive hypercalciuria is due to gastrointestinal hyperabsorption of calcium and is a frequent cause of calcium oxalate nephrolithiasis. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 4.6.1.1; Lyase; Nucleotide Metabolism - purine; Adenylyl cyclase

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: growth cone; cytoskeleton; cell soma; axon; apical part of cell; perinuclear region of cytoplasm; dendrite; cytoplasm; plasma membrane; cytosol

Molecular Function: manganese ion binding; magnesium ion binding; ATP binding; ATPase binding; adenylate cyclase activity

Biological Process: positive regulation of apoptosis; spermatogenesis; cAMP biosynthetic process

Disease: Hypercalciuria, Absorptive, 2