ADARB1 sirna

ADARB1 siRNA (Human)

Cat. Num. AAA8207497

• Gene Names: ADARB1; RED1; ADAR2; DRABA2; DRADA2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADARB1; ADARB1 siRNA (Human); ADAR2; DRADA2; RED1; Double-stranded RNA-specific editase 1; RNA-editing deaminase 1; RNA-editing enzyme 1; dsRNA adenosine deaminase; ADARB1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ADARB1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 674

• Applicable Applications for ADARB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADARB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADARB1 sirna

siRNA to inhibit ADARB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4501919
• NCBI GeneID: 104
• NCBI Accession #: NP_001103.1
• NCBI GenBank Nucleotide #: NM_001112.3
• UniProt Accession #: P78563; O00395; O00465; O00691; O00692; P78555; A6NFK8; A6NJ84; C3TTQ1; C3TTQ2; C9JUP4; G5E9B4
• Molecular Weight: 73,665 Da
• NCBI Official Full Name: double-stranded RNA-specific editase 1 isoform 1
• NCBI Official Synonym Full Names: adenosine deaminase, RNA-specific, B1
• NCBI Official Symbol: ADARB1
• NCBI Official Synonym Symbols: RED1; ADAR2; DRABA2; DRADA2
• NCBI Protein Information: double-stranded RNA-specific editase 1
• UniProt Protein Name: Double-stranded RNA-specific editase 1
• Protein Family: Double-stranded RNA-specific editase
• UniProt Gene Name: ADARB1
• UniProt Synonym Gene Names: ADAR2; DRADA2; RED1
• UniProt Entry Name: RED1_HUMAN

NCBI Description

This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region. [provided by RefSeq, Jul 2008]

Uniprot Description

RED1: Editing of the messenger RNAs for glutamate receptor (GluR) subunits by site-selective adenosine deamination. Edits both the GluR-B Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; RNA-binding; EC 3.5.4.37; Hydrolase

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: adenosine deaminase activity; mRNA binding; double-stranded RNA adenosine deaminase activity; protein binding; RNA binding; metal ion binding; double-stranded RNA binding

Biological Process: RNA processing; negative regulation of cell proliferation; base conversion or substitution editing; positive regulation of viral genome replication; regulation of cell cycle; mRNA modification; innate immune response; gene expression; mRNA processing; negative regulation of cell migration; defense response to virus; adenosine to inosine editing