ADAM8 sirna

ADAM8 siRNA (Human)

Cat. Num. AAA8238313

• Gene Names: ADAM8; MS2; CD156; CD156a
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADAM8; ADAM8 siRNA (Human); MS2; Disintegrin and metalloproteinase domain-containing protein 8; ADAM 8; Cell surface antigen MS2; CD antigen CD156a; ADAM8 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ADAM8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 824

• Applicable Applications for ADAM8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADAM8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADAM8 sirna

siRNA to inhibit ADAM8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 256773264
• NCBI GeneID: 101
• NCBI Accession #: NP_001100.3
• NCBI GenBank Nucleotide #: NM_001109.4
• UniProt Accession #: P78325; B4DVM6; H0YL36; H0YLR0; H0YN39
• Molecular Weight: 80,262 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 8 isoform 1
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 8
• NCBI Official Symbol: ADAM8
• NCBI Official Synonym Symbols: MS2; CD156; CD156a
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 8
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 8
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM8
• UniProt Synonym Gene Names: MS2; ADAM 8
• UniProt Entry Name: ADAM8_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2009]

Uniprot Description

ADAM8: Possible involvement in extravasation of leukocytes.

Protein type: Protease; EC 3.4.24.-; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: specific granule; cell surface; integral to plasma membrane; cytoplasm; plasma membrane; podosome; dense core granule membrane

Molecular Function: protein binding; protein self-association; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; calcium ion binding; cell adhesion molecule binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of cell adhesion; cell morphogenesis; proteolysis; positive regulation of membrane protein ectodomain proteolysis; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; positive regulation of T cell differentiation in the thymus; positive regulation of protein kinase B signaling cascade; extracellular matrix disassembly; cell-cell adhesion; regulation of cell-cell adhesion; positive regulation of protein secretion; positive regulation of acute inflammatory response; positive regulation of bone resorption; negative regulation of neuron apoptosis; angiogenesis; inflammatory response; lymphocyte chemotaxis