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Western Blot (WB) (Western blot analysis of RPL12 expression in Hela (A), SHSY5Y (B), Rat muscle (C) whole cell lysates.)

Rabbit RPL12 Polyclonal Antibody | anti-RPL12 antibody

Anti-RPL12 Antibody

Gene Names
RPL12; L12
Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
RPL12; Polyclonal Antibody; Anti-RPL12 Antibody; 60S ribosomal protein L12; anti-RPL12 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of RPL12 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Sequence Length
165
Applicable Applications for anti-RPL12 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RPL12. The exact sequence is proprietary.
Preparation and Storage
Shipped and store at 4 degree C for one year. Do not freeze.

Western Blot (WB)

(Western blot analysis of RPL12 expression in Hela (A), SHSY5Y (B), Rat muscle (C) whole cell lysates.)

Western Blot (WB) (Western blot analysis of RPL12 expression in Hela (A), SHSY5Y (B), Rat muscle (C) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of RPL12 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of RPL12 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-RPL12 antibody
Rabbit polyclonal antibody to RPL12

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
14,199 Da
NCBI Official Full Name
60S ribosomal protein L12
NCBI Official Synonym Full Names
ribosomal protein L12
NCBI Official Symbol
RPL12
NCBI Official Synonym Symbols
L12
NCBI Protein Information
60S ribosomal protein L12
UniProt Protein Name
60S ribosomal protein L12
Protein Family
UniProt Gene Name
RPL12
UniProt Entry Name
RL12_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L11P family of ribosomal proteins. It is located in the cytoplasm. The protein binds directly to the 26S rRNA. This gene is co-transcribed with the U65 snoRNA, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL12: a ribosomal protein of the L11P family. Located in the cytoplasm. Binds directly to the 26S rRNA. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit.

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: cytosol; focal adhesion; membrane

Molecular Function: protein binding; rRNA binding; structural constituent of ribosome

Biological Process: cellular protein metabolic process; gene expression; mRNA catabolic process, nonsense-mediated decay; ribosomal large subunit assembly and maintenance; selenium metabolic process; selenocysteine metabolic process; SRP-dependent cotranslational protein targeting to membrane; translation; translational elongation; translational initiation; translational termination; viral infectious cycle; viral reproduction; viral transcription

Research Articles on RPL12

Similar Products

Product Notes

The RPL12 rpl12 (Catalog #AAA8243060) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RPL12 Antibody reacts with Human, Mouse, Rat, Bovine, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's RPL12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the RPL12 rpl12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPL12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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