Human ADAM8 PicoKine ELISA Kit | ADAM8 elisa kit

Human ADAM8 PicoKine ELISA Kit

Cat. Num. AAA177612

• Gene Names: ADAM8; MS2; CD156; CD156a
• Reactivity: Human
• Synonyms: ADAM8 PicoKine; Human ADAM8 PicoKine ELISA Kit; Disintegrin and metalloproteinase domain-containing protein 8; A Disintegrin And Metalloproteinase domain 8; ADAM 8; ADAM 8 precursor; ADAM metallopeptidase domain 8; Adam8; ADAM8 protein; ADAM8_HUMAN; CD 156; CD156; CD156a; CD156a antigen; Cell surface antigen MS2; Human leukocyte differentiation antigen; Macrophage cysteine rich glycoprotein; MGC134985; MS 2; MS2 ; ADAM8 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human ADAM8
• Sequence Length: 824

• Assay Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human ADAM8 in cell culture supernates and serum.
• Immunogen: NSO, M1-P497
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human ADAM8 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human ADAM8 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(ng/ml)|0.22|1.47|2.34|0.27|1.56|2.58; Standard deviation|0.013|0.090|0.185|0.018|0.115|0.214; CV(%)|5.7|6.1|7.9|6.8|7.4|8.3
• Sample Data: Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000; O.D.|0.006|0.060|0.129|0.256|0.442|0.829|1.384|1.979
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human ADAM8 PicoKine ELISA Kit standard curve )

Related Product Information for ADAM8 elisa kit

Principle of the assay: Human ADAM8 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM8 has been precoated onto 96-well plates. Standards(NSO, M1-P497) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAM8 amount of sample captured in plate.

Background: A Disintegrin and metalloproteinase domain-containing protein 8 is an enzyme that in humans is encoded by the ADAM8 gene. ADAM8 is localized to chromosome 10q26.3. This gene encodes a member of the ADAM (a disintegrin and metalloproteinase domain) family. Members of the ADAM family, such as ADAM8, are cell surface proteases involved in remodeling of extracellular matrix, cell migration, and processing of membrane-bound signaling molecules. They are characterized by disintegrin and metalloprotease domains that confer adhesive properties and proteolytic activities, respectively. And the protein encoded by this gene may be involved in cell adhesion duringneurodegeneration.

NCBI and Uniprot Product Information

• NCBI GI #: 256773264
• NCBI GeneID: 101
• NCBI Accession #: NP_001100.3
• NCBI GenBank Nucleotide #: NM_001109.4
• UniProt Accession #: P78325; B4DVM6; H0YL36; H0YLR0; H0YN39
• Molecular Weight: 80,262 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 8 isoform 1
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 8
• NCBI Official Symbol: ADAM8
• NCBI Official Synonym Symbols: MS2; CD156; CD156a
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 8
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 8
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM8
• UniProt Synonym Gene Names: MS2; ADAM 8
• UniProt Entry Name: ADAM8_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2009]

Uniprot Description

ADAM8: Possible involvement in extravasation of leukocytes.

Protein type: Protease; Motility/polarity/chemotaxis; Membrane protein, integral; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: cell surface; cytoplasm; dense core granule membrane; integral to plasma membrane; plasma membrane; podosome; specific granule

Molecular Function: calcium ion binding; cell adhesion molecule binding; metalloendopeptidase activity; metallopeptidase activity; protein binding; protein self-association; serine-type endopeptidase activity; zinc ion binding

Biological Process: activation of NF-kappaB transcription factor; angiogenesis; cell morphogenesis; cell-cell adhesion; extracellular matrix disassembly; inflammatory response; leukocyte migration during inflammatory response; lymphocyte chemotaxis; negative regulation of neuron apoptosis; positive regulation of acute inflammatory response; positive regulation of bone resorption; positive regulation of cell adhesion; positive regulation of membrane protein ectodomain proteolysis; positive regulation of protein kinase B signaling cascade; positive regulation of protein secretion; positive regulation of T cell differentiation in the thymus; proteolysis; regulation of cell-cell adhesion