ACOT4 sirna

ACOT4 siRNA (Human)

Cat. Num. AAA8211901

• Gene Names: ACOT4; PTE1B; PTE2B; PTE-Ib
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ACOT4; ACOT4 siRNA (Human); PTE2B; PTEIB; Acyl-coenzyme A thioesterase 4; Acyl-CoA thioesterase 4; PTE-2b; Peroxisomal acyl coenzyme A thioester hydrolase Ib; Peroxisomal long-chain acyl-CoA thioesterase Ib; PTE-Ib; ACOT4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ACOT4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 421

• Applicable Applications for ACOT4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ACOT4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ACOT4 sirna

siRNA to inhibit ACOT4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 187761341
• NCBI GeneID: 122970
• NCBI Accession #: NP_689544.3
• NCBI GenBank Nucleotide #: NM_152331.3
• UniProt Accession #: Q8N9L9; Q17RF4; Q5BKT6; Q86TX0; Q86TX1; Q96N88
• Molecular Weight: 46,327 Da
• NCBI Official Full Name: acyl-coenzyme A thioesterase 4
• NCBI Official Synonym Full Names: acyl-CoA thioesterase 4
• NCBI Official Symbol: ACOT4
• NCBI Official Synonym Symbols: PTE1B; PTE2B; PTE-Ib
• NCBI Protein Information: acyl-coenzyme A thioesterase 4
• UniProt Protein Name: Acyl-coenzyme A thioesterase 4
• Protein Family: Acyl-coenzyme A thioesterase
• UniProt Gene Name: ACOT4
• UniProt Synonym Gene Names: PTE2B; PTEIB; Acyl-CoA thioesterase 4; PTE-Ib
• UniProt Entry Name: ACOT4_HUMAN

Uniprot Description

ACOT4: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Succinyl-CoA thioesterase that also hydrolyzes long chain saturated and unsaturated monocarboxylic acyl-CoAs. Belongs to the C/M/P thioester hydrolase family.

Protein type: Lipid Metabolism - unsaturated fatty acid biosynthesis; Hydrolase; EC 3.1.2.2

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: peroxisome

Molecular Function: acyl-CoA hydrolase activity; succinyl-CoA hydrolase activity; palmitoyl-CoA hydrolase activity; receptor binding

Biological Process: dicarboxylic acid metabolic process; acyl-CoA metabolic process; short-chain fatty acid metabolic process; succinyl-CoA metabolic process; very-long-chain fatty acid metabolic process; saturated monocarboxylic acid metabolic process; long-chain fatty acid metabolic process; dicarboxylic acid catabolic process; unsaturated monocarboxylic acid metabolic process; butyrate metabolic process