ABCB5 sirna

ABCB5 siRNA (Human)

Cat. Num. AAA8230368

• Gene Names: ABCB5; ABCB5beta; EST422562; ABCB5alpha
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ABCB5; ABCB5 siRNA (Human); ATP-binding cassette sub-family B member 5; ABCB5 P-gp; P-glycoprotein ABCB5; ABCB5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ABCB5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1257

• Applicable Applications for ABCB5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ABCB5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ABCB5 sirna

siRNA to inhibit ABCB5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 255708477
• NCBI GeneID: 340273
• NCBI Accession #: NP_001157413.1
• NCBI GenBank Nucleotide #: NM_001163941.1
• UniProt Accession #: Q2M3G0; Q2M3I5; Q5I5Q7; Q5I5Q8; Q6KG50; A4D131; A7BKA4; B5MD19; B7WPL1; F8QQP8; F8QQP9; J3KQ04
• Molecular Weight: 89,832 Da
• NCBI Official Full Name: ATP-binding cassette sub-family B member 5 isoform 1
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family B (MDR/TAP), member 5
• NCBI Official Symbol: ABCB5
• NCBI Official Synonym Symbols: ABCB5beta; EST422562; ABCB5alpha
• NCBI Protein Information: ATP-binding cassette sub-family B member 5
• UniProt Protein Name: ATP-binding cassette sub-family B member 5
• Protein Family: ABC transporter B family
• UniProt Gene Name: ABCB5
• UniProt Entry Name: ABCB5_HUMAN

NCBI Description

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]

Uniprot Description

ABCB5: Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Transporter, ABC family; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7p21.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: efflux transmembrane transporter activity; ATPase activity, coupled to transmembrane movement of substances; ATP binding

Biological Process: regulation of membrane potential; metabolic process; compound eye corneal lens development; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; cell differentiation; transmembrane transport; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent