MYC Associated Factor X Recombinant Protein | MAX recombinant protein

Recombinant MYC Associated Factor X (MAX)

Cat. Num. AAA2030375

• Gene Names: MAX; bHLHd4
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: MYC Associated Factor X; Recombinant MYC Associated Factor X (MAX); MAX recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; SDNDDIEVE SDEEQPRFQS AADKRAHHNA LERKRRDHIK DSFHSLRDSVPSLQGEKASR AQILDKATEY IQYMRRKNHT HQQDIDDLKR QNALLEQQVRALEKARSSAQ LQTNYPSSDN SLYTNAKGST ISAFDGGSDS SSESEPEEPQSRKKLRMEAS
• Sequence Length: 151

• Applicable Applications for MAX recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 48.1kDa
• Accurate Molecular Mass (KD): 48kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and GST-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Ser2~Ser160
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 20379793
• NCBI GeneID: 4149
• Molecular Weight: 9,599 Da
• NCBI Official Full Name: MYC associated factor X
• NCBI Official Synonym Full Names: MYC associated factor X
• NCBI Official Symbol: MAX
• NCBI Official Synonym Symbols: bHLHd4
• NCBI Protein Information: protein max
• UniProt Protein Name: Protein max
• Protein Family: Protein max
• UniProt Gene Name: MAX
• UniProt Synonym Gene Names: BHLHD4; bHLHd4
• UniProt Entry Name: MAX_HUMAN

NCBI Description

The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Mutations of this gene have been reported to be associated with hereditary pheochromocytoma. A pseudogene of this gene is located on the long arm of chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

MAX: a transcription factor. Forms a DNA- binding protein complex with MYC or MAD. The MYC-MAX complex is a transcriptional activator, whereas the MAD-MAX complex is a repressor. CPG methylation of the recognition site greatly inhibits DNA binding, suggesting that DNA methylation may regulate the MYC/MAX complex in vivo. May repress transcription via the recruitment of a chromatin remodeling complex containing H3-K9 histone methyltransferase activity. Three alternatively spliced isoforms have been reported.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 14q23

Cellular Component: nucleoplasm; PML body; cytoplasm; dendrite; nucleus

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; sequence-specific DNA binding; transcription coactivator activity; protein complex binding; transcription cofactor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; neuron apoptosis; regulation of transcription, DNA-dependent; retina development in camera-type eye; protein complex assembly; response to axon injury; cellular response to starvation; response to insulin stimulus