AIFM1 blocking peptide

AIFM1 Blocking Peptide

Cat. Num. AAA823681

• Gene Names: AIFM1; AIF; CMT2D; CMTX4; COWCK; NADMR; NAMSD; PDCD8; COXPD6
• Reactivity: Human, Mouse, Rat, Sheep
• Applications: Blocking (BL)
• Purity: >85%
• Synonyms: AIFM1; AIFM1 Blocking Peptide; AIF; PDCD8; Apoptosis-inducing factor 1; mitochondrial; Programmed cell death protein 8; AIFM1 blocking peptide

• Host: Synthetic
• Reactivity: Human, Mouse, Rat, Sheep
• Purity/Purification: >85%
• Form/Format: Lyophilized powder
• Sequence Length: 261

• Applicable Applications for AIFM1 blocking peptide: Blocking (BL)
• Application Notes: Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
• Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AIFM1 blocking peptide

The peptide is used to block Anti-AIFM1 Antibody reactivity.

NCBI and Uniprot Product Information

• NCBI GI #: 195927004
• NCBI GeneID: 9131
• NCBI Accession #: NP_001124318.1
• NCBI GenBank Nucleotide #: NM_001130846.2
• UniProt Accession #: O95831; Q1L6K4; Q1L6K6; Q2QKE4; Q5JUZ7; Q6I9X6; Q9Y3I3; Q9Y3I4; A4QPB4; B1ALN1; B2RB08; D3DTE9
• Molecular Weight: 26,033 Da
• NCBI Official Full Name: apoptosis-inducing factor 1, mitochondrial isoform 4
• NCBI Official Synonym Full Names: apoptosis-inducing factor, mitochondrion-associated, 1
• NCBI Official Symbol: AIFM1
• NCBI Official Synonym Symbols: AIF; CMT2D; CMTX4; COWCK; NADMR; NAMSD; PDCD8; COXPD6
• NCBI Protein Information: apoptosis-inducing factor 1, mitochondrial; striatal apoptosis-inducing factor; programmed cell death 8 (apoptosis-inducing factor); Neuropathy, axonal motor-sensory, with deafness and mental retardation; neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome)
• UniProt Protein Name: Apoptosis-inducing factor 1, mitochondrial
• UniProt Gene Name: AIFM1
• UniProt Synonym Gene Names: AIF; PDCD8
• UniProt Entry Name: AIFM1_HUMAN

NCBI Description

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, May 2010]

Uniprot Description

PDCD8: Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. Interacts with XIAP/BIRC4. Interacts (via N-terminus) with EIF3G (via C-terminus). Belongs to the FAD-dependent oxidoreductase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Oxidoreductase; EC 1.-.-.-; Mitochondrial

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: mitochondrion; perinuclear region of cytoplasm; mitochondrial inner membrane; mitochondrial intermembrane space; nucleus; cytosol

Molecular Function: protein dimerization activity; protein binding; oxidoreductase activity, acting on NADH or NADPH; DNA binding; electron carrier activity; NAD(P)H oxidase activity

Biological Process: neuron differentiation; caspase activation; chromosome condensation; mitochondrial respiratory chain complex I assembly; neuron apoptosis; apoptosis; cell redox homeostasis; positive regulation of apoptosis; DNA fragmentation during apoptosis; DNA catabolic process

Disease: Cowchock Syndrome