Typical Testing Data/Standard Curve (for reference only)
Guinea Pig CKLFSF8 ELISA Kit | CKLFSF8 elisa kit
Guinea pig CKLFSF8 (Chemokine Like Factor Superfamily 8) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CKLFSF8. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CKLFSF8 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CKLFSF8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CKLFSF8. You can calculate the concentration of CKLFSF8 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the chemokine-like factor gene superfamily, a novel family that is similar to the chemokine and the transmembrane 4 superfamilies. This gene is one of several chemokine-like factor genes located in a cluster on chromosome 3. This gene is widely expressed in many tissues, but the exact function of the encoded protein is unknown. [provided by RefSeq, Jul 2008]
Uniprot Description
CMTM8: a widely distributed protein with four transmembrane regions and a MARVEL domain. Associates with the epidermal growth factor receptor. Transfection into several cell lines suppresses EGF-induced cell proliferation and increases the rate of receptor endocytosis. The lack of EGFR-mediated signaling induces cells to undergo apoptosis via caspase-dependent and -independent pathways. Knockdown of CMTM8 by siRNA promotes cell proliferation and delayed EGFR endocytosis. Two splice-variant human isoforms have been reproted.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3p22.3
Cellular Component: extracellular space; cytoplasm; integral to membrane; nucleus
Molecular Function: cytokine activity
Biological Process: chemotaxis
Research Articles on CKLFSF8
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Product Notes
The Guinea Pig CKLFSF8 cmtm8 (Catalog #AAA2502203) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2502203 ELISA Kit recognizes Guinea Pig CKLFSF8. It is sometimes possible for the material contained within the vial of "CKLFSF8, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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