pNPP 1-Component AP Microwell Substrate | SUBP substrate
pNPP 1-Component AP Microwell Substrate
Synonyms
pNPP 1-Component AP Microwell Substrate; pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets (SUBP); SUBP substrate
Form/Format
Supplied at 1X.
Application Notes
1. Run ELISA according to the specific protocol through the conjugate incubation step.
2. Wash the wells three or four times with 1X ELISA Wash Buffer to remove any residual AP-conjugate.
3. Bring SUBP to room temperature; protect from light.
4. Pipette 100 uL SUBP into each well of the plate.
5. Incubate SUBP 10-60 minutes. Monitor the color intensity.
6. Read the plate between 405-420 nm and analyze. Alternatively, stop the reaction by adding 100 uL/well STOPP and read the samples between 405-420 nm within 1 hour.
2. Wash the wells three or four times with 1X ELISA Wash Buffer to remove any residual AP-conjugate.
3. Bring SUBP to room temperature; protect from light.
4. Pipette 100 uL SUBP into each well of the plate.
5. Incubate SUBP 10-60 minutes. Monitor the color intensity.
6. Read the plate between 405-420 nm and analyze. Alternatively, stop the reaction by adding 100 uL/well STOPP and read the samples between 405-420 nm within 1 hour.
Preparation and Storage
Store at 2-8 degree C. Protected from light.
Related Product Information for SUBP substrate
Description: pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets is a ready-to-use alkaline phosphatase substrate formulation to detect alkaline phosphatase-conjugated molecules in ELISA and microwell assays.
Background: pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets is a ready-to-use alkaline phosphatase substrate formulation to detect alkaline phosphatase-conjugated molecules in ELISA and microwell assays.
Alkaline phosphatase substrates are used in ELISA assays to detect alkaline phosphatase-conjugated molecules via a colorimetric enzymatic reaction. pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets contains para-Nitrophenylphosphate (pNPP). This formulation of pNPP substrate also contains a stabilizer to extend the shelf life of the alkaline phosphatase substrate on your benchtop to 2-3 days.
The alkaline phosphatase substrate reaction may be stopped with the use of a stop solution, Stop Solution for pNPP AP Microwell Substrates.
Background: pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets is a ready-to-use alkaline phosphatase substrate formulation to detect alkaline phosphatase-conjugated molecules in ELISA and microwell assays.
Alkaline phosphatase substrates are used in ELISA assays to detect alkaline phosphatase-conjugated molecules via a colorimetric enzymatic reaction. pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets contains para-Nitrophenylphosphate (pNPP). This formulation of pNPP substrate also contains a stabilizer to extend the shelf life of the alkaline phosphatase substrate on your benchtop to 2-3 days.
The alkaline phosphatase substrate reaction may be stopped with the use of a stop solution, Stop Solution for pNPP AP Microwell Substrates.
Product Categories/Family for SUBP substrate
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Product Notes
The SUBP (Catalog #AAA258075) is a Substrate and is intended for research purposes only. The product is available for immediate purchase. 1. Run ELISA according to the specific protocol through the conjugate incubation step. 2. Wash the wells three or four times with 1X ELISA Wash Buffer to remove any residual AP-conjugate. 3. Bring SUBP to room temperature; protect from light. 4. Pipette 100 uL SUBP into each well of the plate. 5. Incubate SUBP 10-60 minutes. Monitor the color intensity. 6. Read the plate between 405-420 nm and analyze. Alternatively, stop the reaction by adding 100 uL/well STOPP and read the samples between 405-420 nm within 1 hour. Researchers should empirically determine the suitability of the SUBP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "pNPP 1-Component AP Microwell Substrate, Substrate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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