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Western Blot (WB) (Western Blot: Sample: Recombinant RNASE3, Human.)

Rabbit anti-Human Ribonuclease A3 Polyclonal Antibody | anti-RNASE3 antibody

Polyclonal Antibody to Ribonuclease A3 (RNASE3)

Gene Names
RNASE3; ECP; RAF1; RNS3
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Ribonuclease A3; Polyclonal Antibody; Polyclonal Antibody to Ribonuclease A3 (RNASE3); anti-RNASE3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
0.67mg/ml (varies by lot)
Applicable Applications for anti-RNASE3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP).
Application Notes
WB: 0.5 - 2ug/mL
IHC: 5 - 20ug/mL
ICC: 5 - 20ug/mL

Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Cross Reactivity
Human
Traits
Liquid
Immunogen
Recombinant RNASE3 (Pro30~Val154) expressed in E.coli ( MBS2029905 )
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4°C for frequent use.
Aliquot and store at -20°C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant RNASE3, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant RNASE3, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Human Skin cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human RNASE3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ))

Immunohistochemistry (IHC) (DAB staining on IHC-P;Samples: Human Skin cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human RNASE3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ))

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Human Glioma Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human RNASE3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ))

Immunohistochemistry (IHC) (DAB staining on IHC-P;Samples: Human Glioma Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human RNASE3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
eosinophil cationic protein
NCBI Official Synonym Full Names
ribonuclease A family member 3
NCBI Official Symbol
RNASE3
NCBI Official Synonym Symbols
ECP; RAF1; RNS3
NCBI Protein Information
eosinophil cationic protein
UniProt Protein Name
Eosinophil cationic protein
UniProt Gene Name
RNASE3
UniProt Synonym Gene Names
ECP; RNS3; ECP; RNase 3

NCBI Description

The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014]

Uniprot Description

RNASE3: Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. Belongs to the pancreatic ribonuclease family.

Protein type: EC 3.1.27.-; Ribonuclease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular region; extracellular space

Molecular Function: endonuclease activity; lipopolysaccharide binding; nucleic acid binding; ribonuclease activity

Biological Process: antibacterial humoral response; antimicrobial humoral response; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; induction of bacterial agglutination; innate immune response; innate immune response in mucosa; neutrophil degranulation; RNA catabolic process

Research Articles on RNASE3

Similar Products

Product Notes

The RNASE3 rnase3 (Catalog #AAA2026107) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Ribonuclease A3 (RNASE3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ribonuclease A3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP). . WB: 0.5 - 2ug/mL IHC: 5 - 20ug/mL ICC: 5 - 20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the RNASE3 rnase3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ribonuclease A3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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