Western Blot (WB)
((0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Apolipoprotein D Polyclonal Antibody | anti-APOD antibody
Goat anti-apolipoprotein D Antibody
Immunohistochemistry: In paraffin embedded Human Kidney shows staining of the brush border of PCT. Recommended concentration, 5-10ug/ml.
Western Blot: Approx 25kDa band observed in Human Liver and Kidney lysates (calculated MW of 21.3kDa according to NP_001638.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism. [provided by RefSeq, Aug 2008]
Uniprot Description
APOD: APOD occurs in the macromolecular complex with lecithin- cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts. Belongs to the calycin superfamily. Lipocalin family.
Protein type: Lipid-binding; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 3q29
Cellular Component: cell soma; dendrite; endoplasmic reticulum; extracellular region; extracellular space; perinuclear region of cytoplasm
Molecular Function: cholesterol binding; lipid transporter activity; protein binding
Biological Process: aging; angiogenesis; axon regeneration in the peripheral nervous system; brain development; glucose metabolic process; lipid metabolic process; lipid transport; negative regulation of focal adhesion formation; negative regulation of protein import into nucleus; negative regulation of smooth muscle cell proliferation; response to axon injury; response to drug; response to reactive oxygen species; tissue regeneration
Research Articles on APOD
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Product Notes
The APOD apod (Catalog #AAA423239) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-apolipoprotein D Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Apolipoprotein D can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), ELISA (EIA), Immunohitochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Kidney shows staining of the brush border of PCT. Recommended concentration, 5-10ug/ml. Western Blot: Approx 25kDa band observed in Human Liver and Kidney lysates (calculated MW of 21.3kDa according to NP_001638.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the APOD apod for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EIEKIPTTFE NGRC. It is sometimes possible for the material contained within the vial of "Apolipoprotein D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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