STIP1 recombinant protein

STIP1, 1-543aa, Human, E Coli

Cat. Num. AAA206397

• Gene Names: STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: STIP1; 1-543aa; Human; E Coli; Stress-induced-phosphoprotein 1; STI1; HEl-S-94n; HOP; IEF-SSP-3521; P60; STI1L; Epididymis secretory sperm binding protein Li 94n HEL S 94n; Hop Hsc70/Hsp90 organizing protein; IEF SSP 3521 NY REN 11 antigen; P60 Renal carcinoma antigen NY-REN-11 STI1; STIP1_HUMAN Stress induced phosphoprotein 1 Transformation sensitive protein IEF SSP 3521; STIP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MEQVNELKEK GNKALSVGNI DDALQCYSEA IKLDPHNHVL YSNRSAAYAK KGDYQKAYED GCKTVDLKPD WGKGYSRKAA ALEFLNRFEE AKRTYEEGLK HEANNPQLKE GLQNMEARLA ERKFMNPFNM PNLYQKLESD PRTRTLLSDP TYRELIEQLR NKPSDLGTKL QDPRIMTTLS VLLGVDLGSM DEEEEIATPP PPPPPKKETK PEPMEEDLPE NKKQALKEKE LGNDAYKKKD FDTALKHYDK AKELDPTNMT YITNQAAVYF EKGDYNKCRE LCEKAIEVGR ENREDYRQIA KAYARIGNSY FKEEKYKDAI HFYNKSLAEH RTPDVLKKCQ QAEKILKEQE RLAYINPDLA LEEKNKGNEC FQKGDYPQAM KHYTEAIKRN PKDAKLYSNR AACYTKLLEF QLALKDCEEC IQLEPTFIKG YTRKAAALEA MKDYTKAMDV YQKALDLDSS CKEAADGYQR CMMAQYNRHD SPEDVKRRAM ADPEVQQIMS DPAMRLILEQ MQKDPQALSE HLKNPVIAQK IQKLMDVGLI AIR
• Sequence Length: 543

• Applicable Applications for STIP1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for STIP1 recombinant protein

STIP1, also known as stress-induced-phosphoprotein 1 isoform b, belongs to the large group of co-chaperones. The main function of STIP1 is to link Hsp70 and Hsp90 together. STIP1 also modulates the chaperone activities of the linked proteins and possibly interacts with other chaperones and proteins. It forms a complex with HSC70 and HSPCA/HSP-86 and HSPCB/HSP-84, as well as interacting with PACRG. Recombinant human STIP1 was expressed in E Coli and purified by using conventional chromatography techniques

Product Categories/Family for STIP1 recombinant protein

Heat Shock Proteins

References

Johnson BD., et al. (1998) J Biol Chem. 273 (6):3679-86.; Van Der Spuy J., et al. (2001) Protein Expr Purif. 21 (3):462-9.;

NCBI and Uniprot Product Information

• NCBI GI #: 5803181
• NCBI GeneID: 10963
• NCBI Accession #: NP_006810
• NCBI GenBank Nucleotide #: NM_006819.2
• UniProt Accession #: P31948; Q3ZCU9; Q5TZU9; B4DM70; F5H0T1; G3XAD8
• Molecular Weight: 62.6kDa (543aa) confirmed by MALDI-TOF
• NCBI Official Full Name: stress-induced-phosphoprotein 1 isoform b
• NCBI Official Synonym Full Names: stress induced phosphoprotein 1
• NCBI Official Symbol: STIP1
• NCBI Official Synonym Symbols: HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
• NCBI Protein Information: stress-induced-phosphoprotein 1
• UniProt Protein Name: Stress-induced-phosphoprotein 1
• Protein Family: STIP1 homology and U box-containing protein
• UniProt Gene Name: STIP1
• UniProt Synonym Gene Names: STI1; Hop

NCBI Description

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM, Jul 2009]

Uniprot Description

Acts as a co-chaperone for HSP90AA1 (PubMed:27353360). Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90 ().