Rabbit anti-Human, Mouse SULF2 Polyclonal Antibody | anti-SULF2 antibody

SULF2, CT (SULF2, KIAA1247, Extracellular sulfatase Sulf-2) (FITC)

Cat. Num. AAA6352470

• Gene Names: SULF2; HSULF-2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SULF2; Polyclonal Antibody; CT (SULF2; KIAA1247; Extracellular sulfatase Sulf-2) (FITC); Extracellular sulfatase Sulf-2; anti-SULF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SULF2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SULF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 831-860 amino acids from the C-terminal region of human SULF2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SULF2 Antibody (C-term) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the SULF2 antibody detected the SULF2 protein (arrow).)

Western Blot (WB)

(SULF2 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the SULF2 antibody detected the SULF2 protein (arrow).)

Related Product Information for anti-SULF2 antibody

Heparan sulfate proteoglycans (HSPGs) act as coreceptors for numerous heparin-binding growth factors and cytokines and are involved in cell signaling. Heparan sulfate 6-O-endosulfatases, such as SULF2, selectively remove 6-O-sulfate groups from heparan sulfate. This activity modulates the effects of heparan sulfate by altering binding sites for signaling molecules (Dai et al., 2005 [PubMed 16192265]).

Product Categories/Family for anti-SULF2 antibody

Antibodies; KIAA; orf

References

Lai, J.P., et al. Hepatology 52(5):1680-1689(2010). Ellinor, P.T., et al. Nat. Genet. 42(3):240-244(2010). Lemjabbar-Alaoui, H., et al. Oncogene 29(5):635-646(2010). Tang, R., et al. J. Biol. Chem. 284(32):21505-21514(2009). Chau, B.N., et al. Cancer Res. 69(4):1368-1374(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 240255483
• NCBI GeneID: 55959
• NCBI Accession #: NP_001155313.1; NP_061325.1; NP_940998.2
• NCBI GenBank Nucleotide #: NM_001161841.1
• UniProt Accession #: Q8IWU5; Q5JYE1; Q6UX86; Q96SG2; Q9H1H0; Q9UJR3; Q9ULH3; E1P5U6
• Molecular Weight: 100,156 Da
• NCBI Official Full Name: extracellular sulfatase Sulf-2 isoform a
• NCBI Official Synonym Full Names: sulfatase 2
• NCBI Official Symbol: SULF2
• NCBI Official Synonym Symbols: HSULF-2
• NCBI Protein Information: extracellular sulfatase Sulf-2
• UniProt Protein Name: Extracellular sulfatase Sulf-2
• Protein Family: Extracellular sulfatase
• UniProt Gene Name: SULF2
• UniProt Synonym Gene Names: KIAA1247; hSulf-2
• UniProt Entry Name: SULF2_HUMAN

NCBI Description

Heparan sulfate proteoglycans (HSPGs) act as coreceptors for numerous heparin-binding growth factors and cytokines and are involved in cell signaling. Heparan sulfate 6-O-endosulfatases, such as SULF2, selectively remove 6-O-sulfate groups from heparan sulfate. This activity modulates the effects of heparan sulfate by altering binding sites for signaling molecules (Dai et al., 2005 [PubMed 16192265]).[supplied by OMIM, Mar 2008]

Uniprot Description

SULF2: Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Belongs to the sulfatase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Secreted, signal peptide; EC 3.1.6.-; Secreted

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: cell surface; endoplasmic reticulum; extracellular space; Golgi stack; plasma membrane

Molecular Function: arylsulfatase activity; calcium ion binding; N-acetylglucosamine-6-sulfatase activity

Biological Process: cartilage development; chondrocyte development; embryonic skeletal development; esophagus smooth muscle contraction; glomerular basement membrane development; glomerular filtration; heparan sulfate proteoglycan metabolic process; kidney development; negative regulation of fibroblast growth factor receptor signaling pathway; positive regulation of Wnt receptor signaling pathway