Rabbit FOXP1 Polyclonal Antibody | anti-FOXP1 antibody

FOXP1 (Forkhead Box P1, 12CC4, FLJ23741, Forkhead Related Protein-like B, hFKH1B, Glutamine Rich Factor 1, HSPC215, MGC12942, MGC88572, MGC99551, QRF1) (AP)

Cat. Num. AAA6477095

• Gene Names: FOXP1; MFH; QRF1; 12CC4; hFKH1B; HSPC215
• Reactivity: Human, Mouse, Rat
• Applications: Immunocytochemistry, Western Blot, Immunohistochemistry
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: FOXP1; Polyclonal Antibody; FOXP1 (Forkhead Box P1; 12CC4; FLJ23741; Forkhead Related Protein-like B; hFKH1B; Glutamine Rich Factor 1; HSPC215; MGC12942; MGC88572; MGC99551; QRF1) (AP); anti-FOXP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the C-terminus of human FOXP1. Species Crossreactivity: rat, mouse
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-FOXP1 antibody: Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ICC: 1:500; WB: 1ug/ml; IHC: 1:300; Applications are based on unconjugated antibody.
• Immunogen: Linear peptide corresponding to the C-terminus of human FOXP1 (KLH). Species sequence homology: bovine, Rhesus Macaque.
• Conjugate: AP
• Positive Control: Linear peptide corresponding to the C-terminus of human FOXP1.
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunocytochemistry (ICC)

(Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of NIH/3T3, RAW264.7, and HeLa cells using Anti-FOXP1 (Red). Actin filaments have been labeled with AlexaFluor488-Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus, also a little staining in the cytosol.)

Immunohistochemistry (IHC)

(Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded inflammatory skin disorder (Fig 1) and normal human kidney-glomerulus (Fig. 2) tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:300 dilution of Anti-FOXP1. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears in the nucleus.)

Product Categories/Family for anti-FOXP1 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 18644886
• NCBI GeneID: 27086
• NCBI Accession #: NP_116071
• NCBI GenBank Nucleotide #: NM_032682
• UniProt Accession #: Q9H334
• Molecular Weight: 75kDa
• NCBI Official Full Name: forkhead box protein P1 isoform a
• NCBI Official Synonym Full Names: forkhead box P1
• NCBI Official Symbol: FOXP1
• NCBI Official Synonym Symbols: MFH; QRF1; 12CC4; hFKH1B; HSPC215
• NCBI Protein Information: forkhead box protein P1
• UniProt Protein Name: Forkhead box protein P1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXP1
• UniProt Entry Name: FOXP1_HUMAN

NCBI Description

This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding- and protein-protein binding-domains. This gene may act as a tumor suppressor as it is lost in several tumor types and maps to a chromosomal region (3p14.1) reported to contain a tumor suppressor gene(s). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXP1: Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B-cell development. Forms homodimers and heterodimers with FOXP2 and FOXP4. Dimerization is required for DNA-binding. Interacts with CTBP1. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Transcription factor; DNA-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 3p14.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; androgen receptor binding; sequence-specific DNA binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of smooth muscle cell proliferation; response to lipopolysaccharide; osteoclast differentiation; endothelial cell activation; regulation of transcription from RNA polymerase II promoter; macrophage activation; regulation of monocyte differentiation; monocyte activation; regulation of inflammatory response; regulation of interleukin-1 beta secretion; regulation of tumor necrosis factor production; negative regulation of transcription, DNA-dependent; negative regulation of B cell apoptosis

Disease: Mental Retardation With Language Impairment And With Or Without Autistic Features