Typical Testing Data/Standard Curve (for reference only)
StemTAG Alkaline Phosphatase (Purple) Staining Kit
StemTAG Alkaline Phosphatase Staining Kit (Purple)
Synonyms
StemTAG Alkaline Phosphatase (Purple); StemTAG Alkaline Phosphatase Staining Kit (Purple); StemTAG Alkaline Phosphatase (Purple) staining kit
Preparation and Storage
Store all components at 4 degree C until their expiration dates
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for StemTAG Alkaline Phosphatase (Purple) staining kit
Background: Embryonic stem (ES) cells are continuous proliferating stem cell lines of embryonic origin first isolated from the inner cell mass (ICM). Two distinguishing features of ES cells are their ability to be maintained indefinitely in an undifferentiated state and their potential to develop into any cell within the body. Based on previous methods developed for mouse ES cells, human ES cell lines were first established by Dr. James Thomson and colleagues. Like mouse ES cells, human ES cells express high levels of membrane alkaline phosphatase (AP) and Oct-4, a transcriptional factor critical to ICM and germline formation. However, unlike mouse ES cells, hES cells do not express stage-specific embryonic antigen (SSEA-1). In addition, prolonged propagation of hES cells is typically achieved by coculture with primary mouse embryonic fibroblasts (MEFs) serving as feeder cells. Human ES cell lines are not able to maintain their undifferentiated state in the absence of supporting feeder layer cells, even when exogenous cytokines such as leukemia inhibitory factor (LIF) and gelatin-coated plates are used. Although stem cells from different origins require different growth conditions for self-renewal and display different cell surface markers (see Table 1), AP is the most widely used stem cell marker. The StemTAG Alkaline Phosphatase Staining Kit provides an efficient system for monitoring ES cell undifferentiation/ differentiation through AP activity by immunocytochemistry staining.
Product Categories/Family for StemTAG Alkaline Phosphatase (Purple) staining kit
References
1. Wobus AM, Holzhausen H, Jäkel P et al. (1984). Exp Cell Res 152:212-219.
2. Thomson JA, Itskovitz-Eldor J, Shapiro SS et al. (1998). Science 282:1145-1147.
3. Smith AG, Nichols J, Robertson M et al. (1992). Dev Biol 151:339-351.
4. Reubinoff BE, Pera MF, Fong CY et al. (2000). Nat Biotechnol 18:399-404.
2. Thomson JA, Itskovitz-Eldor J, Shapiro SS et al. (1998). Science 282:1145-1147.
3. Smith AG, Nichols J, Robertson M et al. (1992). Dev Biol 151:339-351.
4. Reubinoff BE, Pera MF, Fong CY et al. (2000). Nat Biotechnol 18:399-404.
Similar Products
Product Notes
The StemTAG Alkaline Phosphatase (Purple) (Catalog #AAA168947) is a Staining Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "StemTAG Alkaline Phosphatase (Purple), Staining Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
