Monkey Somatostatin Receptor 1 ELISA Kit | SSTR1 elisa kit

Monkey Somatostatin Receptor 1 ELISA Kit

Cat. Num. AAA740021

• Gene Names: SSTR1; SS1R; SS1-R; SRIF-2; SS-1-R
• Reactivity: Monkey
• Synonyms: Somatostatin Receptor 1; Monkey Somatostatin Receptor 1 ELISA Kit; SSTR1 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This SSTR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey SSTR1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SSTR1 elisa kit

Principle of the Assay: SSTR1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SSTR1. Standards or samples are then added to the microtiter plate wells and SSTR1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SSTR1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for SSTR1 are added to each well to "sandwich" the SSTR1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SSTR1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SSTR1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SSTR1 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 32481993
• NCBI GeneID: 6751
• Molecular Weight: 42,686 Da
• NCBI Official Full Name: somatostatin receptor 1
• NCBI Official Synonym Full Names: somatostatin receptor 1
• NCBI Official Symbol: SSTR1
• NCBI Official Synonym Symbols: SS1R; SS1-R; SRIF-2; SS-1-R
• NCBI Protein Information: somatostatin receptor type 1
• UniProt Protein Name: Somatostatin receptor type 1
• Protein Family: Somatostatin receptor
• UniProt Gene Name: SSTR1
• UniProt Synonym Gene Names: SS-1-R; SS1-R; SS1R
• UniProt Entry Name: SSR1_HUMAN

NCBI Description

Somatostatins are peptide hormones that regulate diverse cellular functions such as neurotransmission, cell proliferation, and endocrine signaling as well as inhibiting the release of many hormones and other secretory proteins. Somatostatin has two active forms of 14 and 28 amino acids. The biological effects of somatostatins are mediated by a family of G-protein coupled somatostatin receptors that are expressed in a tissue-specific manner. The protein encoded by this gene is a member of the superfamily of somatostatin receptors having seven transmembrane segments. Somatostatin receptors form homodimers and heterodimers with other members of the superfamily as well as with other G-protein coupled receptors and receptor tyrosine kinases. This somatostatin receptor has greater affinity for somatostatin-14 than -28. [provided by RefSeq, Jul 2012]

Uniprot Description

SSTR1: Receptor for somatostatin with higher affinity for somatostatin-14 than -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and Na(+)/H(+) exchanger via pertussis toxin insensitive G proteins. Belongs to the G-protein coupled receptor 1 family. Interacts with SKB1.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; Cell cycle regulation; GPCR, family 1

Chromosomal Location of Human Ortholog: 14q13

Cellular Component: neuron projection; integral to plasma membrane; cytoplasm; plasma membrane

Molecular Function: neuropeptide binding; somatostatin receptor activity

Biological Process: response to starvation; negative regulation of cell proliferation; G-protein signaling, coupled to cyclic nucleotide second messenger; synaptic transmission; cell surface receptor linked signal transduction; cell-cell signaling; neuropeptide signaling pathway; glutamate signaling pathway; forebrain development; digestion; cerebellum development; spermatogenesis; response to nutrient