Typical Testing Data/Standard Curve (for reference only)
Porcine Somatostatin Receptor 1 ELISA Kit | SSTR1 elisa kit
Porcine Somatostatin Receptor 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: SSTR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SSTR1 antibody and an SSTR1-HRP conjugate. The assay sample and buffer are incubated together with SSTR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SSTR1 concentration since SSTR1 from samples and SSTR1-HRP conjugate compete for the anti-SSTR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SSTR1 from the sample, fewer sites are left to bind SSTR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SSTR1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Somatostatins are peptide hormones that regulate diverse cellular functions such as neurotransmission, cell proliferation, and endocrine signaling as well as inhibiting the release of many hormones and other secretory proteins. Somatostatin has two active forms of 14 and 28 amino acids. The biological effects of somatostatins are mediated by a family of G-protein coupled somatostatin receptors that are expressed in a tissue-specific manner. The protein encoded by this gene is a member of the superfamily of somatostatin receptors having seven transmembrane segments. Somatostatin receptors form homodimers and heterodimers with other members of the superfamily as well as with other G-protein coupled receptors and receptor tyrosine kinases. This somatostatin receptor has greater affinity for somatostatin-14 than -28. [provided by RefSeq, Jul 2012]
Uniprot Description
SSTR1: Receptor for somatostatin with higher affinity for somatostatin-14 than -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and Na(+)/H(+) exchanger via pertussis toxin insensitive G proteins. Belongs to the G-protein coupled receptor 1 family. Interacts with SKB1.
Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Cell cycle regulation; Membrane protein, integral
Chromosomal Location of Human Ortholog: 14q13
Cellular Component: neuron projection; integral to plasma membrane; cytoplasm; plasma membrane
Molecular Function: neuropeptide binding; somatostatin receptor activity
Biological Process: response to starvation; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of cell proliferation; synaptic transmission; cell surface receptor linked signal transduction; cell-cell signaling; neuropeptide signaling pathway; glutamate signaling pathway; digestion; forebrain development; cerebellum development; spermatogenesis; response to nutrient
Research Articles on SSTR1
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Product Notes
The Porcine SSTR1 sstr1 (Catalog #AAA741047) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA741047 ELISA Kit recognizes Porcine SSTR1. It is sometimes possible for the material contained within the vial of "Somatostatin Receptor 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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