Related Product Information for SST elisa kit
Principle of the Assay: SST ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SST antibody and an SST-HRP conjugate. The assay sample and buffer are incubated together with SST-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SST concentration since SST from samples and SST-HRP conjugate compete for the anti-SST antibody binding site. Since the number of sites is limited, as more sites are occupied by SST from the sample, fewer sites are left to bind SST-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SST concentration in each sample is interpolated from this standard curve.
Product Categories/Family for SST elisa kit
NCBI Description
The hormone somatostatin has active 14 aa and 28 aa forms that are produced by alternate cleavage of the single preproprotein encoded by this gene. Somatostatin is expressed throughout the body and inhibits the release of numerous secondary hormones by binding to high-affinity G-protein-coupled somatostatin receptors. This hormone is an important regulator of the endocrine system through its interactions with pituitary growth hormone, thyroid stimulating hormone, and most hormones of the gastrointestinal tract. Somatostatin also affects rates of neurotransmission in the central nervous system and proliferation of both normal and tumorigenic cells. [provided by RefSeq, Jul 2008]
Uniprot Description
SST: Somatostatin inhibits the release of somatotropin. Belongs to the somatostatin family.
Protein type: Secreted; Secreted, signal peptide; Hormone
Chromosomal Location of Human Ortholog: 3q28
Cellular Component: extracellular space; cell soma; extracellular region
Molecular Function: hormone activity
Biological Process: response to drug; induction of apoptosis by hormones; response to amino acid stimulus; regulation of cell migration; synaptic transmission; negative regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; hyperosmotic response; cell surface receptor linked signal transduction; cell-cell signaling; response to steroid hormone stimulus; response to heat; digestion; response to nutrient
Product Notes
The Canine SST sst (Catalog #
AAA742154) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA742154 ELISA Kit recognizes Canine SST.
It is sometimes possible for the material contained within the vial of
"Somatostatin, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are
absolutely not suitable for use in any
sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a
product from AAA Biotech, you
are explicitly certifying that said products will be properly tested and used in line with
industry standard. AAA Biotech
and its authorized distribution partners reserve the right to refuse to fulfill any order if
we have any indication that a
purchaser may be intending to use a product outside of our accepted criteria.
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aspect of this datasheet at
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of any product performance
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