Rabbit anti-Human SILV Polyclonal Antibody | anti-SILV antibody

SILV, ID (PMEL, D12S53E, PMEL17, SILV, Melanocyte protein PMEL, ME20-M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, M-alpha, 95kD

Cat. Num. AAA6347706

• Gene Names: PMEL; P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: SILV; Polyclonal Antibody; ID (PMEL; D12S53E; PMEL17; Melanocyte protein PMEL; ME20-M; Melanocyte protein Pmel 17; Melanocytes lineage-specific antigen GP100; Melanoma-associated ME20 antigen; P1; P100; Premelanosome protein; Silver locus protein homolog; M-alpha; 95kD; PMEL; 95kD melanocyte-specific secreted glycoprotein; P26; Secreted melanoma-associated ME20 antigen; M-beta; anti-SILV antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SILV.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SILV antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 412-440 in the central region of human SILV.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS642750. This demonstrates that MBS642750 detected the SILV protein (arrow).)

Related Product Information for anti-SILV antibody

SILV plays a central role in the biogenesis of melanosomes. It is involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.

Product Categories/Family for anti-SILV antibody

Antibodies; Cancer Markers

References

1. Leonhardt, R.M., et al. J. Biol. Chem. 285(21):16166-16183(2010). 2. Hoashi, T., et al. FASEB J. 24(3):916-930(2010). 3. Watt, B., et al. J. Biol. Chem. 284(51):35543-35555(2009). 4. McGlinchey, R.P., et al. Proc. Natl. Acad. Sci. U.S.A. 106(33):13731-13736(2009). 5. Kummer, M.P., et al. J. Biol. Chem. 284(4):2296-2306(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 5902084
• NCBI GeneID: 6490
• NCBI Accession #: NP_008859.1
• NCBI GenBank Nucleotide #: NM_006928.4
• UniProt Accession #: P40967; Q12763; Q14448; Q14817; Q16565; B3KS57; B7Z6D7
• Molecular Weight: 70,255 Da
• NCBI Official Full Name: melanocyte protein PMEL isoform 3 preproprotein
• NCBI Official Synonym Full Names: premelanosome protein
• NCBI Official Symbol: PMEL
• NCBI Official Synonym Symbols: P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
• NCBI Protein Information: melanocyte protein PMEL; melanocyte protein mel 17; silver, mouse, homolog of; melanocyte protein Pmel 17; melanosomal matrix protein17; silver locus protein homolog; melanoma-associated ME20 antigen; melanocytes lineage-specific antigen GP100
• UniProt Protein Name: Melanocyte protein PMEL
• Protein Family: Silver-binding protein
• UniProt Gene Name: PMEL
• UniProt Synonym Gene Names: D12S53E; PMEL17; SILV; ME20M; ME20-S; ME20S
• UniProt Entry Name: PMEL_HUMAN

NCBI Description

This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]

Uniprot Description

SILV: Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. Belongs to the PMEL/NMB family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Vesicle

Chromosomal Location of Human Ortholog: 12q13-q14

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; plasma membrane; melanosome; integral to membrane; extracellular region

Molecular Function: protein binding

Biological Process: melanin biosynthetic process; melanosome organization and biogenesis