Bovine Somatostatin ELISA Kit | SST elisa kit

Bovine Somatostatin ELISA Kit

Cat. Num. AAA743278

• Gene Names: SST; SMST
• Reactivity: Bovine
• Synonyms: Somatostatin; Bovine Somatostatin ELISA Kit; SST elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This SST ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine SST. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SST elisa kit

Principle of the Assay: SST ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SST antibody and an SST-HRP conjugate. The assay sample and buffer are incubated together with SST-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SST concentration since SST from samples and SST-HRP conjugate compete for the anti-SST antibody binding site. Since the number of sites is limited, as more sites are occupied by SST from the sample, fewer sites are left to bind SST-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SST concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SST elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 21619156
• NCBI GeneID: 6750
• Molecular Weight: 12,736 Da
• NCBI Official Full Name: Somatostatin
• NCBI Official Synonym Full Names: somatostatin
• NCBI Official Symbol: SST
• NCBI Official Synonym Symbols: SMST
• NCBI Protein Information: somatostatin; somatostatin-14; somatostatin-28; prepro-somatostatin; growth hormone release-inhibiting factor
• UniProt Protein Name: Somatostatin
• Protein Family: Somatostatin
• UniProt Gene Name: SST
• UniProt Entry Name: SMS_HUMAN

NCBI Description

The hormone somatostatin has active 14 aa and 28 aa forms that are produced by alternate cleavage of the single preproprotein encoded by this gene. Somatostatin is expressed throughout the body and inhibits the release of numerous secondary hormones by binding to high-affinity G-protein-coupled somatostatin receptors. This hormone is an important regulator of the endocrine system through its interactions with pituitary growth hormone, thyroid stimulating hormone, and most hormones of the gastrointestinal tract. Somatostatin also affects rates of neurotransmission in the central nervous system and proliferation of both normal and tumorigenic cells. [provided by RefSeq, Jul 2008]

Uniprot Description

SST: Somatostatin inhibits the release of somatotropin. Belongs to the somatostatin family.

Protein type: Secreted; Hormone; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: extracellular space; cell soma; extracellular region

Molecular Function: hormone activity

Biological Process: response to drug; negative regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; synaptic transmission; hyperosmotic response; cell surface receptor linked signal transduction; cell-cell signaling; response to steroid hormone stimulus; response to heat; induction of apoptosis by hormones; digestion; response to amino acid stimulus; regulation of cell migration; response to nutrient