Rabbit anti-Human, Mouse GRIM19 Polyclonal Antibody | anti-GRIM19 antibody

GRIM19 Rabbit pAb

Cat. Num. AAA4760075

• Gene Names: NDUFA13; B16.6; CDA016; CGI-39; GRIM19; GRIM-19
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: GRIM19; Polyclonal Antibody; GRIM19 Rabbit pAb; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex; 13; B16.6; CDA016; CGI-39; GRIM-19; anti-GRIM19 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: 0.7mg/ml (varies by lot)

• Applicable Applications for anti-GRIM19 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:100-1:300
• Immunogen: Fusion protein of human NDUFA13
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of GRIM19 in Mouse skeletal muscle and human hepatocellsular carcinoma tissue in order using GRIM19 Rabbit pAb at dilution 1/350)

Immunohistochemistry (IHC)

(Immunohistochemistry of GRIM19 in paraffin-embedded Human ovarian cancer tissue using GRIM19 Rabbit pAb at dilution 1/50. Predicted cell location: Cytoplasm)

Related Product Information for anti-GRIM19 antibody

This gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. The protein is required for complex I assembly and electron transfer activity. The protein binds the signal transducers and activators of transcription 3 (STAT3) transcription factor, and can function as a tumor suppressor. The human protein purified from mitochondria migrates at approximately 16 kDa. Transcripts originating from an upstream promoter and capable of expressing a protein with a longer N-terminus have been found, but their biological validity has not been determined.

NCBI and Uniprot Product Information

• NCBI GI #: 20139242
• NCBI GeneID: 51079
• UniProt Accession #: Q9P0J0; Q6PKI0; Q9H2L3; Q9Y327; B4DF76; K7EK58
• Molecular Weight: 144
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13
• NCBI Official Symbol: NDUFA13
• NCBI Official Synonym Symbols: B16.6; CDA016; CGI-39; GRIM19; GRIM-19
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13; CI-B16.6; complex I-B16.6; complex I B16.6 subunit; cell death-regulatory protein GRIM19; cell death regulatory protein GRIM-19
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
• UniProt Gene Name: NDUFA13
• UniProt Synonym Gene Names: GRIM19; CI-B16.6; GRIM-19; Gene associated with retinoic and IFN-induced mortality 19 protein
• UniProt Entry Name: NDUAD_HUMAN

NCBI Description

This gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. The protein is required for complex I assembly and electron transfer activity. The protein binds the signal transducers and activators of transcription 3 (STAT3) transcription factor, and can function as a tumor suppressor. The human protein purified from mitochondria migrates at approximately 16 kDa. Transcripts originating from an upstream promoter and capable of expressing a protein with a longer N-terminus have been found, but their biological validity has not been determined. [provided by RefSeq, Oct 2009]

Uniprot Description

GRIM-19: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes. Defects in NDUFA13 may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC). Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms. Belongs to the complex I NDUFA13 subunit family.

Protein type: Oxidoreductase; Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; mitochondrion; mitochondrial membrane; mitochondrial inner membrane; cytoplasm; integral to membrane; mitochondrial respiratory chain; mitochondrial respiratory chain complex I

Molecular Function: protein binding; NADH dehydrogenase (ubiquinone) activity; ATP binding; NADH dehydrogenase activity

Biological Process: cellular metabolic process; positive regulation of protein catabolic process; positive regulation of caspase activity; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; protein import into mitochondrial inner membrane

Disease: Thyroid Carcinoma, Hurthle Cell