Mouse Steroid 5 Alpha Reductase 2 (SRD5a2) ELISA Kit | SRD5a2 elisa kit

Mouse Steroid 5 Alpha Reductase 2 (SRD5a2) ELISA Kit

Cat. Num. AAA2025482

• Reactivity: Mouse
• Synonyms: Steroid 5 Alpha Reductase 2 (SRD5a2); Mouse Steroid 5 Alpha Reductase 2 (SRD5a2) ELISA Kit; 3-Oxo-5 Alpha-Steroid Delta 4-Dehydrogenase Alpha 2; SRD5a2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of SRD5a2. No significant cross-reactivity or interference between SRD5a2 and analogues was observed.
• Sequence Length: 254

• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SRD5a2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SRD5a2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SRD5a2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SRD5a2 in mouse serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to SRD5a2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to SRD5a2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SRD5a2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SRD5a2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SRD5a2 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 338469
• NCBI GeneID: 6716
• Molecular Weight: 28,393 Da
• NCBI Official Full Name: steroid 5-alpha-reductase 2
• NCBI Official Synonym Full Names: steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2)
• NCBI Official Symbol: SRD5A2
• NCBI Protein Information: 3-oxo-5-alpha-steroid 4-dehydrogenase 2
• UniProt Protein Name: 3-oxo-5-alpha-steroid 4-dehydrogenase 2
• Protein Family: 3-oxo-5-alpha-steroid 4-dehydrogenase
• UniProt Gene Name: SRD5A2
• UniProt Synonym Gene Names: S5AR 2
• UniProt Entry Name: S5A2_HUMAN

NCBI Description

This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). [provided by RefSeq, Jul 2008]

Uniprot Description

SRD5A2: Converts testosterone (T) into 5-alpha- dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. Defects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH). A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum. Belongs to the steroid 5-alpha reductase family.

Protein type: Membrane protein, integral; Lipid Metabolism - androgen and estrogen; Oxidoreductase; EC 1.3.1.22; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2p23

Cellular Component: endoplasmic reticulum membrane; cell soma; integral to membrane

Molecular Function: amide binding; cholestenone 5-alpha-reductase activity; sterol 5-alpha reductase activity; 3-oxo-5-alpha-steroid 4-dehydrogenase activity

Biological Process: response to drug; steroid metabolic process; response to nutrient levels; response to peptide hormone stimulus; hypothalamus development; dibenzo-p-dioxin metabolic process; male gonad development; androgen metabolic process; hippocampus development; phthalate metabolic process; androgen biosynthetic process; response to testosterone stimulus; female genitalia development; cell-cell signaling; biphenyl metabolic process; male genitalia development; cell differentiation; response to follicle-stimulating hormone stimulus; steroid catabolic process

Disease: Pseudovaginal Perineoscrotal Hypospadias