Typical Testing Data/Standard Curve (for reference only)
Rat steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) ELISA Kit | SRD5A1 elisa kit
Rat Steroid 5-Alpha-Reductase 1, SRD5A1 ELISA Kit
Gene Names
Srd5a1; S5AR 1
Reactivity
Rat
Synonyms
steroid-5-alpha-reductase; alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1); Rat Steroid 5-Alpha-Reductase 1; SRD5A1 ELISA Kit; Rat Steroid 5-Alpha-Reductase 1 (SRD5A1) ELISA Kit; 3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1; 5-alpha reductase; steroid 5-alpha-reductase type I; steroid-5-alpha-reductase 1; SRD5A1 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat SRD5A1. No significant cross-reactivity or interference between rat SRD5A1 and analogues was observed.
Sequence Length
255
Samples
Serum, plasma, cell culture supernates and tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
15.6 pg/ml-1000 pg/ml
Sensitivity
< 3.9 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SRD5A1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SRD5A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SRD5A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SRD5A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SRD5A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,780 Da
NCBI Official Full Name
3-oxo-5-alpha-steroid 4-dehydrogenase 1
NCBI Official Synonym Full Names
steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
NCBI Official Symbol
Srd5a1
NCBI Official Synonym Symbols
S5AR 1
NCBI Protein Information
3-oxo-5-alpha-steroid 4-dehydrogenase 1; SR type 1; steroid 5 alpha-reductase 1; steroid 5-alpha-reductase 1; steroid-5-alpha-reductase 1
UniProt Protein Name
3-oxo-5-alpha-steroid 4-dehydrogenase 1
Protein Family
UniProt Gene Name
Srd5a1
UniProt Synonym Gene Names
S5AR 1
UniProt Entry Name
S5A1_RAT
NCBI Description
catalyzes the conversion of testosterone to dihydrotestosterone; required for male sex differentiation [RGD, Feb 2006]
Uniprot Description
Function: Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Catalytic activity: A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.
Research Articles on SRD5A1
Similar Products
Product Notes
The Rat SRD5A1 srd5a1 (Catalog #AAA700746) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA700746 ELISA Kit recognizes Rat SRD5A1. It is sometimes possible for the material contained within the vial of "steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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