Typical Testing Data/Standard Curve (for reference only)
Human steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) ELISA Kit | SRD5A1 elisa kit
Human 3-oxo-5-alpha-steroid 4-dehydrogenase 1, SRD5A1 ELISA Kit
Gene Names
SRD5A1; S5AR 1
Reactivity
Human
Synonyms
steroid-5-alpha-reductase; alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1); Human 3-oxo-5-alpha-steroid 4-dehydrogenase 1; SRD5A1 ELISA Kit; Human 3-oxo-5-alpha-steroid 4-dehydrogenase 1 (SRD5A1) ELISA kit; 3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1; 5-alpha reductase; steroid 5-alpha-reductase type I; steroid-5-alpha-reductase 1; SRD5A1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human SRD5A1. No significant cross-reactivity or interference between Human SRD5A1 and analogues was observed.
Sequence Length
259
Samples
Serum, plasma, tissue homogenates and Cell lysates
Assay Type
Sandwich
Detection Range
0.156 ng/ml-10 ng/ml
Sensitivity
0.039 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Developmental Protein
Protein Biological Process 3
Differentiation
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SRD5A1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SRD5A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SRD5A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SRD5A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SRD5A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,459 Da
NCBI Official Full Name
3-oxo-5-alpha-steroid 4-dehydrogenase 1
NCBI Official Synonym Full Names
steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
NCBI Official Symbol
SRD5A1
NCBI Official Synonym Symbols
S5AR 1
NCBI Protein Information
3-oxo-5-alpha-steroid 4-dehydrogenase 1; SR type 1; 5-alpha reductase; steroid 5-alpha-reductase 1; steroid 5-alpha-reductase type I; 3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1
UniProt Protein Name
3-oxo-5-alpha-steroid 4-dehydrogenase 1
Protein Family
UniProt Gene Name
SRD5A1
UniProt Synonym Gene Names
S5AR 1
UniProt Entry Name
S5A1_HUMAN
NCBI Description
Steroid 5-alpha-reductase (EC 1.3.99.5) catalyzes the conversion of testosterone into the more potent androgen, dihydrotestosterone (DHT). Also see SRD5A2 (MIM 607306).[supplied by OMIM, Mar 2008]
Uniprot Description
SRD5A1: Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5- alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. Belongs to the steroid 5-alpha reductase family.
Protein type: EC 1.3.1.22; Membrane protein, integral; Membrane protein, multi-pass; Lipid Metabolism - androgen and estrogen; Oxidoreductase
Chromosomal Location of Human Ortholog: 5p15
Cellular Component: endoplasmic reticulum membrane; integral to membrane
Molecular Function: electron carrier activity; cholestenone 5-alpha-reductase activity; 3-oxo-5-alpha-steroid 4-dehydrogenase activity
Biological Process: sex determination; steroid metabolic process; androgen biosynthetic process; cell differentiation; sex differentiation
Research Articles on SRD5A1
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Product Notes
The Human SRD5A1 srd5a1 (Catalog #AAA915367) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA915367 ELISA Kit recognizes Human SRD5A1. It is sometimes possible for the material contained within the vial of "steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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