Typical Testing Data/Standard Curve (for reference only)
Porcine 3-oxo-5-alpha-steroid 4-dehydrogenase 1 (SRD5A1) ELISA Kit | SRD5A1 elisa kit
Porcine 3-oxo-5-alpha-steroid 4-dehydrogenase 1 (SRD5A1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: sRAGE ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-sRAGE antibody and an sRAGE-HRP conjugate. The assay sample and buffer are incubated together with sRAGE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sRAGE concentration since sRAGE from samples and sRAGE-HRP conjugate compete for the anti-sRAGE antibody binding site. Since the number of sites is limited, as more sites are occupied by sRAGE from the sample, fewer sites are left to bind sRAGE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sRAGE concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Steroid 5-alpha-reductase (EC 1.3.99.5) catalyzes the conversion of testosterone into the more potent androgen, dihydrotestosterone (DHT). Also see SRD5A2 (MIM 607306).[supplied by OMIM, Mar 2008]
Uniprot Description
SRD5A1: Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5- alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. Belongs to the steroid 5-alpha reductase family.
Protein type: Lipid Metabolism - androgen and estrogen; Oxidoreductase; EC 1.3.1.22; Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 5p15
Cellular Component: endoplasmic reticulum membrane; integral to membrane
Molecular Function: electron carrier activity; cholestenone 5-alpha-reductase activity; 3-oxo-5-alpha-steroid 4-dehydrogenase activity
Biological Process: sex determination; steroid metabolic process; androgen biosynthetic process; cell differentiation; sex differentiation
Research Articles on SRD5A1
Similar Products
Product Notes
The Porcine SRD5A1 srd5a1 (Catalog #AAA7242885) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7242885 ELISA Kit recognizes Porcine SRD5A1. It is sometimes possible for the material contained within the vial of "3-oxo-5-alpha-steroid 4-dehydrogenase 1 (SRD5A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
