Alpha-Fodrin (SPTAN1) Recombinant Protein | SPTAN1 recombinant protein

Recombinant Alpha-Fodrin (SPTAN1)

Cat. Num. AAA2009985

• Gene Names: SPTAN1; SPECA
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Alpha-Fodrin (SPTAN1); Recombinant Alpha-Fodrin (SPTAN1); SPTAN1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- AVTQKGNAM VEEGHFAAED VKIKLNELNQ KWDSLKAKAS QRRQDLEDSL QAQQYFADAN EAQSWMREKE PIVGSTDYGK DEDSAEALLK KHEALMSDLS AYGSSIQALR EQAQSCRQQV APTDDETGKE LVLALYDYQE KSPREVTMKK GDILTLLNST NKDWWKVEVN DRQGFVPAAY VKKLDPAQSA SRENLLEEQG SIALRQEQID NQTLITKE
• Sequence Length: 2477

• Applicable Applications for SPTAN1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Chicken (Gallus)
• Expression System: Prokaryotic expression
• Residues: Ala842~Glu1058 (Accession # P07751) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 110227609
• NCBI GeneID: 374234
• NCBI Accession #: NP_001036003.1
• NCBI GenBank Nucleotide #: NM_001042538.1
• UniProt Accession #: P07751
• Molecular Weight: 26.0kDa
• NCBI Official Full Name: spectrin alpha chain, non-erythrocytic 1
• NCBI Official Synonym Full Names: spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
• NCBI Official Symbol: SPTAN1
• NCBI Official Synonym Symbols: SPECA
• NCBI Protein Information: spectrin alpha chain, non-erythrocytic 1; alpha-spectrin; alpha-II spectrin; fodrin alpha chain; nonerythroid alpha-spectrin; spectrin alpha chain, brain; spectrin, non-erythroid alpha chain
• UniProt Protein Name: Spectrin alpha chain, non-erythrocytic 1
• Protein Family: Spectrin
• UniProt Gene Name: SPTAN1
• UniProt Synonym Gene Names: SPTA2
• UniProt Entry Name: SPTN1_CHICK

Uniprot Description

Function: Morphologically, spectrin-like proteins appear to be related to spectrin, showing a flexible rod-like structure. They can bind actin but seem to differ in their calmodulin-binding activity. In nonerythroid tissues, spectrins, in association with some other proteins, may play an important role in membrane organization.

Subunit structure: Like erythrocyte spectrin, the spectrin-like proteins are capable of forming dimers which can further associate to tetramers. Interacts with ACP1

By similarity.

Subcellular location: Cytoplasm › cytoskeleton. Cytoplasm › cell cortex.

Domain: Spectrin-like proteins have five domains: (1) N-terminal domain (N), (2) domain between the N-terminal and middle domain (NM), (3) middle domain (M), (4) domain between the middle and C-terminal domain (MC), (5) C-terminal domain (C). NM and MC domains are composed of typical spectrin 106 residues repeats (1-8 for NM and 12-19 for MC) and are homologous to each other. N, M, and C domains are composed of sequences that do not form typical spectrin repeats.

Post-translational modification: Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro

By similarity.

Sequence similarities: Belongs to the spectrin family.Contains 3 EF-hand domains.Contains 1 SH3 domain.Contains 20 spectrin repeats.