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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Gallus Alpha-Fodrin (SPTAN1) Polyclonal Antibody | anti-SPTAN1 antibody

Polyclonal Antibody to Alpha-Fodrin (SPTAN1)

Gene Names
SPTAN1; SPECA
Reactivity
Gallus
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Alpha-Fodrin (SPTAN1); Polyclonal Antibody; Polyclonal Antibody to Alpha-Fodrin (SPTAN1); anti-SPTAN1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Gallus
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against SPTAN1. It has been selected for its ability to recognize SPTAN1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- E NITAKMKGLK GKVSDLEKAA AQRKAKLDEN SAFLQFNWKA DVVESWIGEK ENSLKTDDYG RDLSSVQTLL TKQETFDAGL QAFQQEGIAN ITALKDQLLA AKHIQSKAIE VRHASLMKRW NQLLANSAAR KKKLLEAQEH FRKVEDLFLT FAKKASAFNS WFENAEEDLT DPVRCNSLEE IKALREA
Sequence Length
920
Applicable Applications for anti-SPTAN1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant SPTAN1 (Glu1950~Ala2137) expressed in E.coli.
Cross Reactivity
Chicken
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2040571
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
285,363 Da
NCBI Official Full Name
spectrin alpha chain, non-erythrocytic 1
NCBI Official Synonym Full Names
spectrin alpha, non-erythrocytic 1
NCBI Official Symbol
SPTAN1
NCBI Official Synonym Symbols
SPECA
NCBI Protein Information
spectrin alpha chain, non-erythrocytic 1
UniProt Protein Name
Spectrin alpha chain, non-erythrocytic 1
Protein Family
UniProt Gene Name
SPTAN1
UniProt Synonym Gene Names
SPTA2

Uniprot Description

Morphologically, spectrin-like proteins appear to be related to spectrin, showing a flexible rod-like structure. They can bind actin but seem to differ in their calmodulin-binding activity. In nonerythroid tissues, spectrins, in association with some other proteins, may play an important role in membrane organization.

Research Articles on SPTAN1

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Product Notes

The SPTAN1 sptan1 (Catalog #AAA2005044) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Alpha-Fodrin (SPTAN1) reacts with Gallus and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha-Fodrin (SPTAN1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the SPTAN1 sptan1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- E NITAKMKGLK GKVSDLEKAA AQRKAKLDEN SAFLQFNWKA DVVESWIGEK ENSLKTDDYG RDLSSVQTLL TKQETFDAGL QAFQQEGIAN ITALKDQLLA AKHIQSKAIE VRHASLMKRW NQLLANSAAR KKKLLEAQEH FRKVEDLFLT FAKKASAFNS WFENAEEDLT DPVRCNSLEE IKALREA. It is sometimes possible for the material contained within the vial of "Alpha-Fodrin (SPTAN1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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