SDS-Page
Alpha-Fodrin (SPTAN1) Recombinant Protein | SPTAN1 recombinant protein
Recombinant Alpha-Fodrin (SPTAN1)
Gene Names
Sptan1; A2a; IPF; Spna2
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Alpha-Fodrin (SPTAN1); Recombinant Alpha-Fodrin (SPTAN1); SPTAN1 recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- NSATRKKKLL EAQSHFRKVE DLFLTFAKKA SAFNSWFENA EEDLTDPVRC NSLEEIKALR EAHDAFRSSL SSAQADFNQL AELDRQIKSF RVASNPYTWF TMEALEETWR NLQKIIKERE LELQKEQRRQ EENDKLRQEF AQHANAFHQW IQETRTYLLD GSCMVEESGT LESQLEATKR KHQEIRAMRS QLKKIEDLG
MGHHHHHHSGSEF- NSATRKKKLL EAQSHFRKVE DLFLTFAKKA SAFNSWFENA EEDLTDPVRC NSLEEIKALR EAHDAFRSSL SSAQADFNQL AELDRQIKSF RVASNPYTWF TMEALEETWR NLQKIIKERE LELQKEQRRQ EENDKLRQEF AQHANAFHQW IQETRTYLLD GSCMVEESGT LESQLEATKR KHQEIRAMRS QLKKIEDLG
Sequence Length
2472
Applicable Applications for SPTAN1 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Rattus norvegicus (Rat)
Expression System
Prokaryotic expression
Residues
Asn2071~Gly2269 (Accession # P16086) with N-terminal His-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25.0kDa
NCBI Official Full Name
spectrin alpha chain, non-erythrocytic 1
NCBI Official Synonym Full Names
spectrin, alpha, non-erythrocytic 1
NCBI Official Symbol
Sptan1
NCBI Official Synonym Symbols
A2a; IPF; Spna2
NCBI Protein Information
spectrin alpha chain, non-erythrocytic 1; a--fodrin; alpha-fodrin; alpha-spectrin 2; alpha II spectrin; alpha-II spectrin; fodrin alpha chain; brain alpha-spectrin; inhibitory protein factor; spectrin alpha chain, brain; noerythroid alpha-spectrin 2; nonerythroid alpha-spectrin 2; spectrin, non-erythroid alpha chain
UniProt Protein Name
Spectrin alpha chain, non-erythrocytic 1
Protein Family
UniProt Gene Name
Sptan1
UniProt Synonym Gene Names
Spna2; Spta2
UniProt Entry Name
SPTN1_RAT
NCBI Description
a filamentous protein that plays an important role in membrane organization [RGD, Feb 2006]
Uniprot Description
SPTAN1: a cytoskeletal protein which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. Capable to forming dimers which can further associate to tetramers.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Adaptor/scaffold
Cellular Component: cortical cytoskeleton; protein complex; intracellular membrane-bound organelle; paranode region of axon; fascia adherens; cytosol; Z disc; microtubule cytoskeleton; spectrin; membrane; plasma membrane; lateral plasma membrane; vesicle; myelin sheath
Molecular Function: calmodulin binding; protein binding; syntaxin binding; spectrin binding; protein heterodimerization activity; protein complex binding; protein N-terminus binding; calcium ion binding; actin binding
Biological Process: actin filament capping; actin cytoskeleton reorganization
Research Articles on SPTAN1
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Product Notes
The SPTAN1 sptan1 (Catalog #AAA2012109) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Alpha-Fodrin (SPTAN1) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the SPTAN1 sptan1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF- NSATRKKKLL EAQSHFRKVE DLFLTFAKKA SAFNSWFENA EEDLTDPVRC NSLEEIKALR EAHDAFRSSL SSAQADFNQL AELDRQIKSF RVASNPYTWF TMEALEETWR NLQKIIKERE LELQKEQRRQ EENDKLRQEF AQHANAFHQW IQETRTYLLD GSCMVEESGT LESQLEATKR KHQEIRAMRS QLKKIEDLG. It is sometimes possible for the material contained within the vial of "Alpha-Fodrin (SPTAN1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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