Typical Testing Data/Standard Curve (for reference only)
(Mouse OPN PicoKine ELISA Kit standard curve )
Mouse OPN ELISA Kit | SPP1 elisa kit
Mouse OPN PicoKine ELISA Kit
Gene Names
Spp1; OP; 2AR; Bsp; Eta; Opn; Ric; BNSP; BSPI; Opnl; Apl-1; ETA-1; Spp-1
Reactivity
Mouse
Synonyms
OPN; Mouse OPN PicoKine ELISA Kit; Osteopontin; secreted phosphoprotein 1; BNSP; Bone sialoprotein 1; Bone sialoprotein I; BSPI; Early T lymphocyte activation 1; ETA 1; MGC110940; Nephropontin; OSTP_HUMAN; Secreted phosphoprotein 1; Secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1); Secreted phosphoprotein 1 (osteopontin bone sialoprotein); SPP-1; SPP1; Urinary stone protein; Uropontin; SPP1 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse OPN
Sequence Length
294
Samples
Cell culture supernates, serum, plasma (heparin, EDTA) and urine
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 10pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse OPN PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse OPN PicoKine ELISA Kit standard curve )
Related Product Information for SPP1 elisa kit
Principle of the assay: mouse OPN ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for OPN has been precoated onto 96-well plates. Standards(NSO,L17-N294) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for OPN is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse OPN amount of sample captured in plate.
Background: Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extracellular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extracellular matrix, allowing them to perform their respective functions during osteogenesis.1Osteopontin is presumably involved in stone formation as stone matrix.2 The deduced protein sequence reveals a317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4.3 The standard product used in this kit is recombinant mouse OPN, consistingof 284 amino acid sequence with the molecular mass of 31.5KDa. As a result of glycosylation, the molecular mass is 65KDa.
Background: Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extracellular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extracellular matrix, allowing them to perform their respective functions during osteogenesis.1Osteopontin is presumably involved in stone formation as stone matrix.2 The deduced protein sequence reveals a317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4.3 The standard product used in this kit is recombinant mouse OPN, consistingof 284 amino acid sequence with the molecular mass of 31.5KDa. As a result of glycosylation, the molecular mass is 65KDa.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
32,429 Da
NCBI Official Full Name
Bone marrow macrophage cDNA, RIKEN full-length enriched library, clone:I830015P03 product:secreted phosphoprotein 1, full insert sequence
NCBI Official Synonym Full Names
secreted phosphoprotein 1
NCBI Official Symbol
Spp1
NCBI Official Synonym Symbols
OP; 2AR; Bsp; Eta; Opn; Ric; BNSP; BSPI; Opnl; Apl-1; ETA-1; Spp-1
NCBI Protein Information
osteopontin; minopontin; bone sialoprotein 1; osteopontin-like protein; 44 kDa bone phosphoprotein; early T-lymphocyte activation 1 protein; calcium oxalate crystal growth inhibitor protein
UniProt Protein Name
Putative uncharacterized protein
Protein Family
UniProt Gene Name
Spp1
UniProt Entry Name
Q3UBR1_MOUSE
Uniprot Description
osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular space; cell projection; apical part of cell; perinuclear region of cytoplasm; cytoplasm; extracellular region
Molecular Function: extracellular matrix binding; cytokine activity
Biological Process: response to organic substance; osteoblast differentiation; neutrophil chemotaxis; ossification; response to vitamin D; response to steroid hormone stimulus; biomineral formation; positive regulation of bone resorption; cell adhesion; negative regulation of apoptosis
Research Articles on SPP1
Similar Products
Product Notes
The Mouse SPP1 spp1 (Catalog #AAA175823) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA175823 ELISA Kit recognizes Mouse SPP1. It is sometimes possible for the material contained within the vial of "OPN, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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