Sphingosine Kinase 1 Phospho-Regulation Antibody Sampler Kit | SPHK1K antibody sampler kit
Sphingosine Kinase 1 Phospho-Regulation
Reactivity
Human, Rat, Mouse, Chicken
Applications
Western Blot, ELISA, Immunocytochemistry
Synonyms
Sphingosine Kinase 1 Phospho-Regulation; SPHK1K antibody sampler kit
Reactivity
Human, Rat, Mouse, Chicken
Form/Format
Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide, and the peptides are supplied in PBS and 0.05% sodium azi
Applicable Applications for SPHK1K antibody sampler kit
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
Preparation and Storage
Store all at -20 degree C. Stable for 1 year.
Related Product Information for SPHK1K antibody sampler kit
Kit Summary: The sphingosine kinase 1 (SK1) phospho-regulation antibody sampler kit can be used to detect the level ofphosphorylation of SK1 (Tyr-225) relative to the total expression level of SK1. The kit also includes peptides for antibodyblocking experiments and a secondary reagent for antibody detection.
Background: Sphingolipids are metabolized into bioactive products that include ceramide, sphingosine, and sphingosine-1-phosphate.SK catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P).S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cellproliferation, survival, motility, and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA forboth of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen, and lung, while SK2 is highest in kidney and liver. SK can be activated through growth factor, G protein-coupled, andimmunoglobulin receptor signaling. SK1 has been shown to mediate cell growth, prevention of apoptosis, and cellulartransformation, and is upregulated in a variety of human tumors. Regulation of SK1 may occur through ERK mediatedphosphorylation of Ser-225. This phosphorylation leads to increased activity and translocation to the plasma membrane.
Background: Sphingolipids are metabolized into bioactive products that include ceramide, sphingosine, and sphingosine-1-phosphate.SK catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P).S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cellproliferation, survival, motility, and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA forboth of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen, and lung, while SK2 is highest in kidney and liver. SK can be activated through growth factor, G protein-coupled, andimmunoglobulin receptor signaling. SK1 has been shown to mediate cell growth, prevention of apoptosis, and cellulartransformation, and is upregulated in a variety of human tumors. Regulation of SK1 may occur through ERK mediatedphosphorylation of Ser-225. This phosphorylation leads to increased activity and translocation to the plasma membrane.
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Product Notes
The Human, Rat, Mouse, Chicken SPHK1K (Catalog #AAA474564) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474564 Antibody Sampler Kit recognizes Human, Rat, Mouse, Chicken SPHK1K. AAA Biotech's Sphingosine Kinase 1 Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the SPHK1K for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sphingosine Kinase 1 Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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