Typical Testing Data/Standard Curve
Human Hevin ELISA Kit | SPARCL1 elisa kit
Human Hevin ELISA Kit
Gene Names
SPARCL1; SC1; MAST9; PIG33; MAST 9
Reactivity
Human
Applications
ELISA
Synonyms
Hevin; Human Hevin ELISA Kit; SPARC-like protein 1; High endothelial venule protein; MAST 9; SPARCL1 elisa kit
Reactivity
Human
Specificity
The Human Hevin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Hevin proteins within the range of 46.9 pg/ml - 3000 pg/ml.
Sequence Length
664
Applicable Applications for SPARCL1 elisa kit
Sandwich ELISA (EIA)
Samples
Cell Culture Supernatants, Serum.
Sensitivity
10 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for SPARCL1 elisa kit
Principle of the Assay||The Human Hevin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Hevin in Cell Culture Supernatants, Serum. This assay employs an antibody specific for Human Hevin coated on a 96-well plate. Standards and samples are pipetted into the wells and Hevin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Hevin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Hevin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. SPARCL1 is highly expressed in HEV and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.
Background/Introduction: SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. SPARCL1 is highly expressed in HEV and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
61,760 Da
NCBI Official Full Name
SPARC-like protein 1
NCBI Official Synonym Full Names
SPARC like 1
NCBI Official Symbol
SPARCL1
NCBI Official Synonym Symbols
SC1; MAST9; PIG33; MAST 9
NCBI Protein Information
SPARC-like protein 1
UniProt Protein Name
SPARC-like protein 1
Protein Family
UniProt Gene Name
SPARCL1
UniProt Synonym Gene Names
Hevin
UniProt Entry Name
SPRL1_HUMAN
Uniprot Description
SPARCL1: Belongs to the SPARC family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4q22.1
Cellular Component: extracellular space; proteinaceous extracellular matrix
Molecular Function: calcium ion binding
Biological Process: signal transduction
Research Articles on SPARCL1
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Product Notes
The Human SPARCL1 sparcl1 (Catalog #AAA8244608) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8244608 ELISA Kit recognizes Human SPARCL1. AAA Biotech's Hevin can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (EIA). Researchers should empirically determine the suitability of the SPARCL1 sparcl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hevin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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