Osteonectin/SPARC/BM-40 Recombinant Protein | SPARC/BM-40 recombinant protein

Recombinant Human Osteonectin/SPARC/BM-40 Protein (C-6His)

Cat. Num. AAA2553037

• Gene Names: SPARC; ON; OI17; BM-40
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Osteonectin/SPARC/BM-40; Recombinant Human Osteonectin/SPARC/BM-40 Protein (C-6His); SPARC; Basement-Membrane Protein 40; BM-40; Osteonectin; ON; Secreted Protein Acidic and Rich in Cysteine; SPARC/BM-40 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Sequence Positions: Ala18-Ile303
• Sequence Length: 302

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fusion Tag: C-6His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for SPARC/BM-40 recombinant protein

Secreted Protein Acidic and Rich in Cysteine (SPARC) is a secreted, evolutionarily conserved collagen-binding glycoprotein and belongs to the SPARC family. SPARC has 286 amino acids and contains an EF-hand in C-termina domain, a follistatin-like domain with Kazal-like sequences. There are two calcium binding sites, one binds 5 - 8 Ca2+ with a low affinity and other on an EF-hand loop that binds a Ca2+ ion with a high affinity. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC regulate cell growth through interactions with the extracellular matrix (ECM) and cytokines. SPARC bind to numerous proteins of the ECM, affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC also binds several types of collagen, albumin, thrombospondin, PDGF and cell membranes.

NCBI and Uniprot Product Information

• NCBI GI #: 827342585
• NCBI GeneID: 6678
• NCBI Accession #: NP_001296372.1
• NCBI GenBank Nucleotide #: NM_001309443.1
• UniProt Accession #: P09486; Q6IBK4; D3DQH9
• Molecular Weight: Molecular Mass: 33.7 kDa; Actual Protein Molecular Mass: 36 kDa
• NCBI Official Full Name: SPARC isoform 2
• NCBI Official Synonym Full Names: secreted protein acidic and cysteine rich
• NCBI Official Symbol: SPARC
• NCBI Official Synonym Symbols: ON; OI17; BM-40
• NCBI Protein Information: SPARC
• UniProt Protein Name: SPARC
• UniProt Gene Name: SPARC
• UniProt Synonym Gene Names: ON; BM-40; ON

NCBI Description

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]

Uniprot Description

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.