Rabbit anti-Human COCH Polyclonal Antibody | anti-COCH antibody

COCH, CT (COCH, COCH5B2, Cochlin, COCH-5B2) (PE)

Cat. Num. AAA6287492

• Gene Names: COCH; DFNA9; COCH5B2; DFNB110; COCH-5B2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: COCH; Polyclonal Antibody; CT (COCH; COCH5B2; Cochlin; COCH-5B2) (PE); COCH-5B2; anti-COCH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 550

• Applicable Applications for anti-COCH antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: COCH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 499-528 amino acids from the C-terminal region of human COCH.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(COCH Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the COCH antibody detected the COCH protein (arrow).)

Western Blot (WB)

(Western blot analysis of COCH (arrow) using rabbit polyclonal COCH Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the COCH gene.)

Related Product Information for anti-COCH antibody

The protein encoded by this gene is highly conserved in human, mouse, and chicken, showing 94% and 79% amino acid identity of human to mouse and chicken sequences, respectively. Hybridization to this gene was detected in spindle-shaped cells located along nerve fibers between the auditory ganglion and sensory epithelium. These cells accompany neurites at the habenula perforata, the opening through which neurites extend to innervate hair cells. This and the pattern of expression of this gene in chicken inner ear paralleled the histologic findings of acidophilic deposits, consistent with mucopolysaccharide ground substance, in temporal bones from DFNA9 (autosomal dominant nonsyndromic sensorineural deafness 9) patients. Mutations that cause DFNA9 have been reported in this gene. Alternative splicing results in multiple transcript variants encoding the same protein. Additional splice variants encoding distinct isoforms have been described but their biological validities have not been demonstrated. [provided by RefSeq].

Product Categories/Family for anti-COCH antibody

Antibodies; Proteins

References

Ikezono, T., et al. Acta Otolaryngol. 130(8):881-887(2010). Yao, J., et al. J. Biol. Chem. 285(20):14909-14919(2010). Baek, J.I., et al. Clin. Genet. 77(4):399-403(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010). Lee, E.S., et al. Invest. Ophthalmol. Vis. Sci. 51(4):2060-2066(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4758022
• NCBI GeneID: 1690
• NCBI Accession #: NP_004077.1
• NCBI GenBank Nucleotide #: NP_004077.1
• UniProt Accession #: O43405
• NCBI Official Full Name: cochlin isoform b
• NCBI Official Synonym Full Names: cochlin
• NCBI Official Symbol: COCH
• NCBI Official Synonym Symbols: DFNA9; COCH5B2; DFNB110; COCH-5B2
• NCBI Protein Information: cochlin
• UniProt Protein Name: Cochlin
• Protein Family: Cochlin
• UniProt Gene Name: COCH
• UniProt Synonym Gene Names: COCH5B2
• UniProt Entry Name: COCH_HUMAN

NCBI Description

The protein encoded by this gene is highly conserved in human, mouse, and chicken, showing 94% and 79% amino acid identity of human to mouse and chicken sequences, respectively. Hybridization to this gene was detected in spindle-shaped cells located along nerve fibers between the auditory ganglion and sensory epithelium. These cells accompany neurites at the habenula perforata, the opening through which neurites extend to innervate hair cells. This and the pattern of expression of this gene in chicken inner ear paralleled the histologic findings of acidophilic deposits, consistent with mucopolysaccharide ground substance, in temporal bones from DFNA9 (autosomal dominant nonsyndromic sensorineural deafness 9) patients. Mutations that cause DFNA9 have been reported in this gene. Alternative splicing results in multiple transcript variants encoding the same protein. Additional splice variants encoding distinct isoforms have been described but their biological validities have not been demonstrated. [provided by RefSeq, Oct 2008]