Sperm-associated antigen 8 (SPAG8) Recombinant Protein | SPAG8 recombinant protein

Recombinant Human Sperm-associated antigen 8 (SPAG8)

Cat. Num. AAA1346026

• Gene Names: SPAG8; SMP1; BS-84; CT142; HSD-1; SPAG3; CILD28; hSMP-1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Sperm-associated antigen 8 (SPAG8); Recombinant Human Sperm-associated antigen 8 (SPAG8); SPAG8 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-426, Full length protein
• Sequence: METNESTEGSRSRSRSLDIQPSSEGLGPTSEPFPSSDDSPRSALAAATAAAAAAASAAAATAAFTTAKAAALSTKTPAPCSEFMEPSSDPSLLGEPCAGPGFTHNIAHGSLGFEPVYVSCIAQDTCTTTDHSSNPGPVPGSSSGPVLGSSSGAGHGSGSGSGPGCGSVPGSGSGPGPGSGPGSGPGHGSGSHPGPASGPGPDTGPDSELSPCIPPGFRNLVADRVPNYTSWSQHCPWEPQKQPPWEFLQVLEPGARGLWKPPDIKGKLMVCYETLPRGQCLLYNWEEERATNHLDQVPSMQDGSESFFFRHGHRGLLTMQLKSPMPSSTTQKDSYQPPGNVYWPLRGKREAMLEMLLQHQICKEVQAEQEPTRKLFEVESVTHHDYRMELAQAGTPAPTKPHDYRQEQPETFWIQRAPQLPVCEGD
• Sequence Length: 426

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for SPAG8 recombinant protein

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved identification and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. This protein is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.

NCBI and Uniprot Product Information

• NCBI GI #: 88853066
• NCBI GeneID: 26206
• NCBI Accession #: NP_001034681.1
• NCBI GenBank Nucleotide #: NM_001039592.1
• UniProt Accession #: Q99932; Q12937; Q5TCV8; Q8WWB4; B4DY89; E9PDV6
• Molecular Weight: 51,139 Da
• NCBI Official Full Name: sperm-associated antigen 8 isoform 1
• NCBI Official Synonym Full Names: sperm associated antigen 8
• NCBI Official Symbol: SPAG8
• NCBI Official Synonym Symbols: SMP1; BS-84; CT142; HSD-1; SPAG3; CILD28; hSMP-1
• NCBI Protein Information: sperm-associated antigen 8
• UniProt Protein Name: Sperm-associated antigen 8
• Protein Family: Sperm-associated antigen
• UniProt Gene Name: SPAG8
• UniProt Synonym Gene Names: SMP-1

NCBI Description

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. This protein interacts with ACT (activator of CREM in testis) and may play a role in CREM (cAMP response element modulator)-ACT-mediated gene transcription during spermatogenesis. This protein may also play a role in spermatogenesis by regulating microtubule formation and cell division. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined. [provided by RefSeq, Jul 2012]

Uniprot Description

Plays a role in spermatogenesis by enhancing the binding of CREM isoform tau to its coactivator FHL5 and increasing the FHL5-regulated transcriptional activation of CREM isoform tau (). Involved in the acrosome reaction and in binding of sperm to the zona pellucida (). Plays a role in regulation of the cell cycle by controlling progression through the G2/M phase, possibly by delaying the activation of CDK1 which is required for entry into mitosis (PubMed:19548270). May play a role in fertility and microtubule formation through interaction with RANBP9 (PubMed:10500252).