SPA17 recombinant protein

SPA17, 1-151aa, Human, His tag, E Coli

Cat. Num. AAA205194

• Gene Names: SPA17; CT22; SP17; SP17-1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: SPA17; 1-151aa; Human; His tag; E Coli; Sperm surface protein Sp17; CT22; SP17; SP17-1; SPA17 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSIPFSN THYRIPQGFG NLLEGLTREI LREQPDNIPA FAAAYFESLL EKREKTNFDP AEWGSKVEDR FYNNHAFEEQ EPPEKSDPKQ EESQISGKEE ETSVTILDSS EEDKEKEEVA AVKIQAAFRG HIAREEAKKM KTNSLQNEEK EENK
• Sequence Length: 151

• Applicable Applications for SPA17 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for SPA17 recombinant protein

SPA17 is a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. Recombinant human SPA17 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for SPA17 recombinant protein

Cancer

References

Buchli R., et al. (2002) Biochim. Biophys. Acta. 1578:29-42; Li Z., et al. (2007) Int. J. Cancer. 121:1507-1511;

NCBI and Uniprot Product Information

• NCBI GI #: 8394343
• NCBI GeneID: 53340
• NCBI Accession #: NP_059121
• NCBI GenBank Nucleotide #: NM_017425.3
• UniProt Accession #: Q15506; Q9BXF7; B2R4F2
• Molecular Weight: 19.8 kDa (174aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
• NCBI Official Full Name: sperm surface protein Sp17
• NCBI Official Synonym Full Names: sperm autoantigenic protein 17
• NCBI Official Symbol: SPA17
• NCBI Official Synonym Symbols: CT22; SP17; SP17-1
• NCBI Protein Information: sperm surface protein Sp17
• UniProt Protein Name: Sperm surface protein Sp17
• Protein Family: Sperm surface protein
• UniProt Gene Name: SPA17
• UniProt Synonym Gene Names: SP17; CT22; Sperm protein 17

NCBI Description

This gene encodes a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. A retrotransposed pseudogene is present on chromosome 10q22.[provided by RefSeq, Jan 2009]

Uniprot Description

Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates ().