Human desmin, Des ELISA Kit | Des elisa kit

Human desmin, Des ELISA Kit

Cat. Num. AAA160424

• Gene Names: DES; CSM1; CSM2; CDCD3; CMD1F; LGMD1D; LGMD1E; LGMD2R
• Reactivity: Human
• Synonyms: desmin; Des; Human desmin; Des ELISA Kit; Des elisa kit

• Reactivity: Human
• Sequence Length: 470

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.5ng/ml - 60ng/ml
• Sensitivity: 0.21ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Des elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Desmin (also known as DES) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human DES antibody. DES present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human DES Antibody is added and binds to DES in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DES antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human DES. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 55749932
• NCBI GeneID: 1674
• NCBI Accession #: NP_001918.3
• NCBI GenBank Nucleotide #: NM_001927.3
• UniProt Accession #: P17661; Q15787; Q549R7; Q549R8; Q549R9; Q8IZR1; Q8IZR6; Q8NES2; Q8NEU6; Q8TAC4; Q8TCX2; Q8TD99
• Molecular Weight: 53,536 Da
• NCBI Official Full Name: desmin
• NCBI Official Synonym Full Names: desmin
• NCBI Official Symbol: DES
• NCBI Official Synonym Symbols: CSM1; CSM2; CDCD3; CMD1F; LGMD1D; LGMD1E; LGMD2R
• NCBI Protein Information: desmin
• UniProt Protein Name: Desmin
• Protein Family: Desmin
• UniProt Gene Name: DES

NCBI Description

This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008]

Uniprot Description

Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed:25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190). May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin ().